Multi-dimensional liquid chromatography in proteomics—a review
Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …
functions. This still-emerging combination of technologies aims to describe and characterize …
Recent advances on multidimensional liquid chromatography–mass spectrometry for proteomics: From qualitative to quantitative analysis—A review
Q Wu, H Yuan, L Zhang, Y Zhang - Analytica chimica acta, 2012 - Elsevier
With the acceleration of proteome research, increasing attention has been paid to
multidimensional liquid chromatography–mass spectrometry (MDLC–MS) due to its high …
multidimensional liquid chromatography–mass spectrometry (MDLC–MS) due to its high …
Off-line multidimensional liquid chromatography and auto sampling result in sample loss in LC/LC–MS/MS
S Magdeldin, JJ Moresco, T Yamamoto… - Journal of proteome …, 2014 - ACS Publications
Large-scale proteomics often employs two orthogonal separation methods to fractionate
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …
Recent development of multi-dimensional chromatography strategies in proteome research
As a complementary approach to two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE), multi-dimensional chromatography separation methods have been widely applied in …
PAGE), multi-dimensional chromatography separation methods have been widely applied in …
Recent advances in peptide separation by multidimensional liquid chromatography for proteome analysis
S Di Palma, ML Hennrich, AJR Heck, S Mohammed - Journal of proteomics, 2012 - Elsevier
Shotgun proteomics dominates the field of proteomics. The foundations of the strategy
consist of multiple rounds of peptide separation where chromatography provides the …
consist of multiple rounds of peptide separation where chromatography provides the …
Multidimensional separations for protein/peptide analysis in the post-genomic era
H Liu, D Lin, JR Yates III - Biotechniques, 2002 - Taylor & Francis
Proteomics is the study of all or part of the protein complement of genes in an organism,
often involving the analysis of complex protein/peptide samples. Such complex samples are …
often involving the analysis of complex protein/peptide samples. Such complex samples are …
Highly efficient peptide separations in proteomics: Part 2: Bi-and multidimensional liquid-based separation techniques
Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …
resolution of the enormous number of peptides generated upon tryptic digestion of …
Multidimensional chromatography coupled to mass spectrometry in analysing complex proteomics samples
P Horvatovich, B Hoekman… - Journal of separation …, 2010 - Wiley Online Library
Multidimensional chromatography coupled to mass spectrometry (LCn‐MS) provides more
separation power and an extended measured dynamic concentration range to analyse …
separation power and an extended measured dynamic concentration range to analyse …
Multidimensional separation of peptides for effective proteomic analysis
HJ Issaq, KC Chan, GM Janini, TP Conrads… - … of Chromatography B, 2005 - Elsevier
Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …
digestion of a protein mixture followed by separation using multidimensional liquid …
Role of chromatographic techniques in proteomic analysis
I Neverova, JE Van Eyk - Journal of Chromatography B, 2005 - Elsevier
Proteomics, the characterization of the proteome, is conceptually simple but technically
challenging. Development of such technologies as mass spectrometry, multidimensional …
challenging. Development of such technologies as mass spectrometry, multidimensional …