A nomenclature for fungal PCR primers with examples from intron-containing SSU rDNA
A Gargas, PT DePriest - Mycologia, 1996 - Taylor & Francis
We present a compilation of polymerase chain reaction primers (oligonucleotides) used to
amplify and sequence the small subunit of nuclear ribosomal DNA. To simplify use of these …
amplify and sequence the small subunit of nuclear ribosomal DNA. To simplify use of these …
Limits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi
L Kiss - Proceedings of the National Academy of Sciences, 2012 - National Acad Sciences
In PNAS, the work by Schoch et al.(1) proposed nuclear ribosomal DNA internal transcribed
spacer (ITS) sequences as the sole universal barcode for fungi. The work by Schoch et al.(1) …
spacer (ITS) sequences as the sole universal barcode for fungi. The work by Schoch et al.(1) …
Phylogenetic comparison of protein-coding versus ribosomal RNA-coding sequence data: a case study of the Lecanoromycetes (Ascomycota)
V Hofstetter, J Miadlikowska, F Kauff… - Molecular phylogenetics …, 2007 - Elsevier
The resolving power and statistical support provided by two protein-coding (RPB1 and
RPB2) and three ribosomal RNA-coding (nucSSU, nucLSU, and mitSSU) genes individually …
RPB2) and three ribosomal RNA-coding (nucSSU, nucLSU, and mitSSU) genes individually …
Making and sequencing heavily multiplexed, high-throughput 16S ribosomal RNA gene amplicon libraries using a flexible, two-stage PCR protocol
A Naqib, S Poggi, W Wang, M Hyde… - … analysis: Methods and …, 2018 - Springer
Deep sequencing of polymerase chain reaction (PCR)-amplified small subunit (16S or 18S)
ribosomal RNA (rRNA) genes fragments is commonly employed to characterize the …
ribosomal RNA (rRNA) genes fragments is commonly employed to characterize the …
Partial phylogeny of the unicellular eukaryotes based on rapid sequencing of a portion of 28S ribosomal RNA.
A Baroin, R Perasso, LH Qu… - Proceedings of the …, 1988 - National Acad Sciences
Using a rapid rRNA sequencing technique, we have determined the sequence of the 400
nucleotides located at the 5'end of the large subunit rRNA molecule from eight species of …
nucleotides located at the 5'end of the large subunit rRNA molecule from eight species of …
[图书][B] Ribosomal RNA analysis of microorganisms as they occur in nature
DM Ward, MM Bateson, R Weller, AL Ruff-Roberts - 1992 - Springer
Advances in molecular biology are now providing the means for solving long-standing
problems in microbiology. One of the best examples is the development of a rational …
problems in microbiology. One of the best examples is the development of a rational …
Diversity of soil fungi studied by PCR–RFLP of ITS
M Viaud, A Pasquier, Y Brygoo - Mycological Research, 2000 - cambridge.org
Ribosomal DNA sequences provide new molecular approaches to characterise fungal
species from the environment. This study presents a culture-independent approach to …
species from the environment. This study presents a culture-independent approach to …
Fungal-specific PCR primers developed for analysis of the ITS region of environmental DNA extracts
KJ Martin, PT Rygiewicz - BMC microbiology, 2005 - Springer
Abstract Background The Internal Transcribed Spacer (ITS) regions of fungal ribosomal DNA
(rDNA) are highly variable sequences of great importance in distinguishing fungal species …
(rDNA) are highly variable sequences of great importance in distinguishing fungal species …
Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots
The first DNA sequences obtained from arbuscular endomycorrhizal fungi are reported.
They were obtained by directly sequencing overlapping amplified fragments of the nuclear …
They were obtained by directly sequencing overlapping amplified fragments of the nuclear …
Ribosomal RNA genes in eukaryotic microorganisms: witnesses of phylogeny?
AL Torres-Machorro, R Hernández… - FEMS microbiology …, 2010 - academic.oup.com
The study of genomic organization and regulatory elements of rRNA genes in metazoan
paradigmatic organisms has led to the most accepted model of rRNA gene organization in …
paradigmatic organisms has led to the most accepted model of rRNA gene organization in …