Design and experimental application of a novel non-degenerate universal primer set that amplifies prokaryotic 16S rRNA genes with a low possibility to amplify …

H Mori, F Maruyama, H Kato, A Toyoda… - DNA …, 2014 - academic.oup.com
The deep sequencing of 16S rRNA genes amplified by universal primers has revolutionized
our understanding of microbial communities by allowing the characterization of the diversity …

Phylogenetic utility of indels within ribosomal DNA and β-tubulin sequences from fungi in the Rhizoctonia solani species complex

D González, MA Cubeta, R Vilgalys - Molecular Phylogenetics and …, 2006 - Elsevier
The genus Rhizoctonia consists of a diverse assemblage of anamorphic fungi frequently
associated with plants and soil throughout the world. Some anamorphs are related with …

Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit.

YJ Liu, S Whelen, BD Hall - Molecular biology and evolution, 1999 - academic.oup.com
In an effort to establish a suitable alternative to the widely used 18S rRNA system for
molecular systematics of fungi, we examined the nuclear gene RPB2, encoding the second …

The ribosomal database project (RDP)

BL Maidak, GJ Olsen, N Larsen… - Nucleic acids …, 1996 - academic.oup.com
Abstract The Ribosomal Database Project (RDP) is a curated database that offers ribosome-
related data, analysis services and associated computer programs. The offerings include …

Fungal identification using molecular tools: a primer for the natural products research community

HA Raja, AN Miller, CJ Pearce… - Journal of natural …, 2017 - ACS Publications
Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They
are known to produce numerous bioactive molecules, which makes them very useful for …

[引用][C] Direct ribosomal RNA sequencing: optimization of extraction and sequencing methods for work with higher plants

R Keith Hamby, L Sims, L Issel, E Zimmer - Plant Molecular Biology …, 1988 - Springer
R NA sequencing with reverse transcriptase, synthetic oligonucleotide primers and
dideoxynucleotides is a particularly attractive choice for comparing ribosomal RNAs …

Identifying the 'unidentified'fungi: a global-scale long-read third-generation sequencing approach

L Tedersoo, S Anslan, M Bahram, U Kõljalg… - Fungal Diversity, 2020 - Springer
Molecular identification methods, in particular high-throughput sequencing tools, have
greatly improved our knowledge about fungal diversity and biogeography, but many of the …

Interpretation of PCR methods for species definition

PD Bridge, DK Arora - Applications of PCR in Mycology, 1998 - books.google.com
The introduction of molecular biological techniques has been a major force in the areas of
systematics and population biology of filamentous fungi. Unlike the development of …

The evolutionary position of the rhodophytePorphyra umbilicalis and the basidiomyceteLeucosporidium scottii among other eukaryotes as deduced from complete …

L Hendriks, R De Baere, Y Van de Peer, J Neefs… - Journal of Molecular …, 1991 - Springer
The complete small ribosomal subunit RNA (srRNA) sequence was determined for the red
alga Porphyra umbilicalis and the basidiomycete Leucosporidium scottii, representing two …

High-density microarray of small-subunit ribosomal DNA probes

KH Wilson, WJ Wilson, JL Radosevich… - Applied and …, 2002 - Am Soc Microbiol
Ribosomal DNA sequence analysis, originally conceived as a way to provide a universal
phylogeny for life forms, has proven useful in many areas of biological research. Some of the …