From Molecules to Classrooms: A Comprehensive Guide to Single-Molecule Localization Microscopy

G Yan, Y Zhang, A Allamprese, KN Brooks… - Journal of Chemical …, 2024 - ACS Publications
Single-molecule localization microscopy (SMLM) has revolutionized our ability to visualize
cellular structures, offering unprecedented detail. However, the intricate biophysical …

Raw data to results: a hands-on introduction and overview of computational analysis for single-molecule localization microscopy

KJA Martens, B Turkowyd, U Endesfelder - Frontiers in Bioinformatics, 2022 - frontiersin.org
Single-molecule localization microscopy (SMLM) is an advanced microscopy method that
uses the blinking of fluorescent molecules to determine the position of these molecules with …

Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Machine Learning for Single-Molecule Localization Microscopy: From Data Analysis to Quantification

J Liu, Y Li, T Chen, F Zhang, F Xu - Analytical Chemistry, 2024 - ACS Publications
Single-molecule localization microscopy (SMLM) is a versatile tool for realizing nanoscale
imaging with visible light and providing unprecedented opportunities to observe …

Spectroscopic single-molecule localization microscopy: applications and prospective

B Brenner, C Sun, FM Raymo, HF Zhang - Nano convergence, 2023 - Springer
Single-molecule localization microscopy (SMLM) breaks the optical diffraction limit by
numerically localizing sparse fluorescence emitters to achieve super-resolution imaging …

About samples, giving examples: optimized single molecule localization microscopy

A Jimenez, K Friedl, C Leterrier - Methods, 2020 - Elsevier
Super-resolution microscopy has profoundly transformed how we study the architecture of
cells, revealing unknown structures and refining our view of cellular assemblies. Among the …

Single-molecule localization super-resolution microscopy: deeper and faster

S Herbert, H Soares, C Zimmer… - Microscopy and …, 2012 - academic.oup.com
For over a decade fluorescence microscopy has demonstrated the capacity to achieve
single-molecule localization accuracies of a few nanometers, well below the∼ 200 nm …

Build and operation of a custom 3D, multicolor, single-molecule localization microscope

RM Power, A Tschanz, T Zimmermann, J Ries - Nature Protocols, 2024 - nature.com
Single-molecule localization microscopy (SMLM) enables imaging scientists to visualize
biological structures with unprecedented resolution. Particularly powerful implementations of …

Multicolor single-molecule localization microscopy: review and prospect

X Chen, X Wang, F Huang, D Ma - PhotoniX, 2024 - Springer
Single-molecule localization microscopy (SMLM) surpasses the diffraction limit by randomly
switching fluorophores between fluorescent and dark states, precisely pinpointing the …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …