The multicellular tumor spheroid model is a widely used culture system to mimic the three-
dimensionality of tumors. Several methods and an even larger number of protocols are …

To obtain a multicellular MCF-7 spheroid model to mimic the three-dimensional (3D) of
tumors, the microwell liquid overlay (A) and hanging-drop/agar (B) methods were first …

Multicellular tumor spheroids are widely used models in tumor research. Because of their
three dimensional organization they can simulate avascular tumor areas comprising …

Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid
tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent …

Abstract Background Traditional two-dimensional (2-D) monolayer cell culture is vastly
different from in vivo physiological conditions, which can lead to inaccurate or insufficient …

The purpose of the meeting was to exchange information on spheroid culture methods and
to review the role of spheroids in cancer research. Techniques and results presented at this …

Tumor spheroids are three-dimensional clusters of cancer cells that exhibit characteristics of
poorly perfused tumors and hence present a relevant model for testing the efficacy of anti …

Conventional two dimensional (2D) monolayer cell culture has been considered the 'gold
standard'technique for in vitro cellular experiments. However, the need for a model that …

Spheroids are widely used in biology because they provide an in vitro 3-dimensional (3D)
model to study proliferation, cell death, differentiation, and metabolism of cells in tumors and …

The growth of tumour cells as three‐dimensional multicellular spheroids in vitro has led to
important insights in tumour biology, since properties of the in vivo‐tumour such as …