[HTML][HTML] Gene length and detection bias in single cell RNA sequencing protocols
Background: Single cell RNA sequencing (scRNA-seq) has rapidly gained popularity for
profiling transcriptomes of hundreds to thousands of single cells. This technology has led to …
profiling transcriptomes of hundreds to thousands of single cells. This technology has led to …
[HTML][HTML] Assessing characteristics of RNA amplification methods for single cell RNA sequencing
HR Dueck, R Ai, A Camarena, B Ding, R Dominguez… - BMC genomics, 2016 - Springer
Background Recently, measurement of RNA at single cell resolution has yielded surprising
insights. Methods for single-cell RNA sequencing (scRNA-seq) have received considerable …
insights. Methods for single-cell RNA sequencing (scRNA-seq) have received considerable …
[HTML][HTML] Comparative analysis of single-cell RNA sequencing methods
Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and
medical questions. However, systematic comparisons of the performance of diverse scRNA …
medical questions. However, systematic comparisons of the performance of diverse scRNA …
Power analysis of single-cell RNA-sequencing experiments
Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method
to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and …
to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and …
[HTML][HTML] Experimental considerations for single-cell RNA sequencing approaches
QH Nguyen, N Pervolarakis, K Nee… - Frontiers in cell and …, 2018 - frontiersin.org
Single-cell transcriptomic technologies have emerged as powerful tools to explore cellular
heterogeneity at the resolution of individual cells. Previous scientific knowledge in cell …
heterogeneity at the resolution of individual cells. Previous scientific knowledge in cell …
[HTML][HTML] Batch effects and the effective design of single-cell gene expression studies
Single-cell RNA sequencing (scRNA-seq) can be used to characterize variation in gene
expression levels at high resolution. However, the sources of experimental noise in scRNA …
expression levels at high resolution. However, the sources of experimental noise in scRNA …
Introduction to single‐cell RNA sequencing
TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …
Single-cell RNA sequencing analysis: a step-by-step overview
Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …
individual cells can now be measured for thousands of cells in a single experiment. Since its …
Systematic comparative analysis of single cell RNA-sequencing methods
J Ding, X Adiconis, SK Simmons, MS Kowalczyk… - BioRxiv, 2019 - biorxiv.org
ABSTRACT A multitude of single-cell RNA sequencing methods have been developed in
recent years, with dramatic advances in scale and power, and enabling major discoveries …
recent years, with dramatic advances in scale and power, and enabling major discoveries …
Approaches for single-cell RNA sequencing across tissues and cell types
Single-cell sequencing of RNA (scRNA-seq) has advanced our understanding of cellular
heterogeneity and signaling in developmental biology and disease. A large number of …
heterogeneity and signaling in developmental biology and disease. A large number of …