Acinetobacter baylyi ADP1: Transforming the choice of model organism

KT Elliott, EL Neidle - IUBMB life, 2011 - Wiley Online Library
For more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology
studies that address a broad range of questions. Recently, the rapid accumulation of data …

Acinetobacter baylyi ADP1—naturally competent for synthetic biology

S Santala, V Santala - Essays in Biochemistry, 2021 - portlandpress.com
Acinetobacter baylyi ADP1 is a non-pathogenic soil bacterium known for its metabolic
diversity and high natural transformation and recombination efficiency. For these features, A …

Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution

BA Renda, A Dasgupta, D Leon… - Journal of bacteriology, 2015 - Am Soc Microbiol
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic
biology because it is naturally transformable. To examine the genetic reliability of this …

Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx

GA Suárez, BA Renda, A Dasgupta… - Applied and …, 2017 - Am Soc Microbiol
The genomes of most bacteria contain mobile DNA elements that can contribute to
undesirable genetic instability in engineered cells. In particular, transposable insertion …

Natural transformation as a tool in Acinetobacter baylyi: streamlined engineering and mutational analysis

SR Bedore, EL Neidle, I Pardo, J Luo, AC Baugh… - Methods in …, 2023 - Elsevier
Natural transformation and homologous recombination in a soil bacterium, Acinetobacter
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …

Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering

D Metzgar, JM Bacher, V Pezo, J Reader… - Nucleic acids …, 2004 - academic.oup.com
Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …

Expression vectors for Acinetobacter baylyi ADP1

CD Murin, K Segal, A Bryksin… - Applied and …, 2012 - Am Soc Microbiol
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …

Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1

BW Biggs, SR Bedore, E Arvay, S Huang… - Nucleic Acids …, 2020 - academic.oup.com
One primary objective of synthetic biology is to improve the sustainability of chemical
manufacturing. Naturally occurring biological systems can utilize a variety of carbon sources …

A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1

V De Berardinis, D Vallenet, V Castelli… - Molecular systems …, 2008 - embopress.org
We have constructed a collection of single‐gene deletion mutants for all dispensable genes
of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were …

Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining

GA Suárez, KR Dugan, BA Renda… - Nucleic Acids …, 2020 - academic.oup.com
One goal of synthetic biology is to improve the efficiency and predictability of living cells by
removing extraneous genes from their genomes. We demonstrate improved methods for …