Acinetobacter baylyi ADP1: Transforming the choice of model organism
KT Elliott, EL Neidle - IUBMB life, 2011 - Wiley Online Library
For more than 25 years, Acinetobacter baylyi ADP1 has been used in molecular biology
studies that address a broad range of questions. Recently, the rapid accumulation of data …
studies that address a broad range of questions. Recently, the rapid accumulation of data …
Acinetobacter baylyi ADP1—naturally competent for synthetic biology
Acinetobacter baylyi ADP1 is a non-pathogenic soil bacterium known for its metabolic
diversity and high natural transformation and recombination efficiency. For these features, A …
diversity and high natural transformation and recombination efficiency. For these features, A …
Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic
biology because it is naturally transformable. To examine the genetic reliability of this …
biology because it is naturally transformable. To examine the genetic reliability of this …
Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx
The genomes of most bacteria contain mobile DNA elements that can contribute to
undesirable genetic instability in engineered cells. In particular, transposable insertion …
undesirable genetic instability in engineered cells. In particular, transposable insertion …
Natural transformation as a tool in Acinetobacter baylyi: streamlined engineering and mutational analysis
Natural transformation and homologous recombination in a soil bacterium, Acinetobacter
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …
Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering
D Metzgar, JM Bacher, V Pezo, J Reader… - Nucleic acids …, 2004 - academic.oup.com
Acinetobacter sp. strain ADP1 is a naturally transformable gram-negative bacterium with
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …
simple culture requirements, a prototrophic metabolism and a compact genome of 3.7 Mb …
Expression vectors for Acinetobacter baylyi ADP1
CD Murin, K Segal, A Bryksin… - Applied and …, 2012 - Am Soc Microbiol
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …
recombination, so it can be transformed without restriction digests or ligation reactions …
Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1
One primary objective of synthetic biology is to improve the sustainability of chemical
manufacturing. Naturally occurring biological systems can utilize a variety of carbon sources …
manufacturing. Naturally occurring biological systems can utilize a variety of carbon sources …
A complete collection of single‐gene deletion mutants of Acinetobacter baylyi ADP1
V De Berardinis, D Vallenet, V Castelli… - Molecular systems …, 2008 - embopress.org
We have constructed a collection of single‐gene deletion mutants for all dispensable genes
of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were …
of the soil bacterium Acinetobacter baylyi ADP1. A total of 2594 deletion mutants were …
Rapid and assured genetic engineering methods applied to Acinetobacter baylyi ADP1 genome streamlining
One goal of synthetic biology is to improve the efficiency and predictability of living cells by
removing extraneous genes from their genomes. We demonstrate improved methods for …
removing extraneous genes from their genomes. We demonstrate improved methods for …