Analysis of pea chloroplast inner and outer envelope membrane proteins by two-dimensional gel electrophoresis and their comparison with stromal proteins

M Werner-Washburne, K Cline, K Keegstra - Plant physiology, 1983 - academic.oup.com
Abstract Analysis of inner and outer pea (Pisum sativum var. Laxtons Progress No. 9)
chloroplast envelope membranes by sodium dodecyl sulfate-polyacrylamide gel …

Proteins and polypeptides of envelope membranes from spinach chloroplasts. I. Isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis …

TD Nguyen - Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1983 - Elsevier
Polypeptides of spinach chloroplast envelopes were separated by electrophoresis in an
SDS-polyacrylamide gradient gel. At least 37 polypeptides were resolved; nine were …

Electrophoretic characterization of membrane proteins during chloroplast development in barley

NC Nielsen - European journal of biochemistry, 1975 - Wiley Online Library
Membranes of plastids isolated from greening 15‐cm (6 days) barley seedling were
analysed electro‐phoretically using acid‐soaked polyacrylamide gels. During greening five …

Visualization of contact sites between outer and inner envelope membranes in isolated chloroplasts

FFM Cremers, WF Voorhout, TP van der Krift… - … et Biophysica Acta (BBA …, 1988 - Elsevier
Contact sites between outer and inner envelope membranes of isolated chloroplasts have
been studied at the ultrastructural level by two different electron microscopical methods, ie …

Outer envelope membranes from chloroplasts are isolated as right-side-out vesicles

K Waegemann, S Eichacker, J Soll - Planta, 1992 - Springer
Outer envelope membranes were isolated from purified chloroplasts of pea leaves. The
sidedness of the vesicles was analyzed by (i) aqueous polymer-two phase partitioning,(ii) …

Preparation and characterization of membrane fractions enriched in outer and inner envelope membranes from spinach chloroplasts. I. Electrophoretic and …

MA Block, A Dorne, J Joyard, R Douce - Journal of Biological Chemistry, 1983 - ASBMB
We have developed a fast and reliable method for the separation of two membrane fractions
respectively enriched in outer and inner envelope membranes from isolated, intact, purified …

Isolation and characterization of chloroplast envelope membranes

K Keegstra, AE Yousif - Methods in enzymology, 1986 - Elsevier
Publisher Summary This chapter discusses the isolation and characterization of chloroplast
envelope membranes. The chloroplast envelope consists of a pair of lipid bilayer …

Polypeptide composition of envelopes of spinach chloroplasts: Two major proteins occupy 90% of outer envelope membranes

H Koike, M Yoshio, Y Kashino… - Plant and cell …, 1998 - academic.oup.com
Outer and inner envelope membranes of spinach chloroplasts were isolated using floatation
centrifugation followed by sedimentation sucrose density gradient centrifugation after …

Polypeptide pattern and enzymic character of vacuoles isolated from barley mesophyll protoplasts

G Kaiser, E Martinoia, JM Schmitt, DK Hincha, U Heber - Planta, 1986 - Springer
Intact chloroplasts and vacuoles were isolated from mesophyll protoplasts of barley. The
chloroplasts occupied about 15% of the cellular volume and contained 75% of the protein …

Comparative Studies of the Thylakoid Proteins of Mesophyll and Bundle Sheath Plastids of Zea mays

SJ Kirchanski, RB Park - Plant Physiology, 1976 - academic.oup.com
The proteins from both grana and stroma lamellae of maize (Zea mays) mesophyll plastids
and from maize bundle sheath plastid membranes have been compared by electrophoresis …