Celery is one of the most important vegetable crop and its yield and quality is influenced by
many environmental factors. Researches on gene expression not only help to unravel the …

Due to its accuracy, sensitivity and high throughput, real time quantitative PCR (RT-qPCR)
has been widely used in analysing gene expression. The quality of data from such analyses …

RT-qPCR is a widely used method in gene expression and transcriptome studies.
Normalization based on reference genes is necessary to accurately analyze RT-qPCR data …

Iris. lactea var. chinensis (I. lactea var. chinensis) is highly tolerant of Cu, Pb, drought, NaCl
and cold. To study the molecular mechanism of I. lactea var. chinensis under these stresses …

Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops
in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A …

The process of selection and validation of reference genes is the first step in studies of gene
expression by real-time quantitative polymerase chain reaction (RT-qPCR). The genome of …

Background Real-time quantitative PCR (RT-qPCR) is the most commonly used method for
accurately detecting gene expression patterns. As part of RT-qPCR analysis, normalization …

Selecting suitable internal reference genes is an important prerequisite for the application of
quantitative real-time PCR (qRT-PCR). However, no systematic studies have been …

Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular
technique for investigating gene expression levels in plants. Based on the Minimum …

Licorice (Glycyrrhiza glabra L.) is an important medicinal plant accumulating high-value
secondary metabolites. Real-time reverse transcription quantitative PCR (RT-qPCR) has …