Improving the spatial resolution of optical microscopes is important for a vast number of
applications in the life sciences. Optical microscopy allows intact samples and living cells to …

During the last decade, various efforts have been undertaken to enhance the resolution of
optical microscopes, mostly because of their importance in biological sciences. Herein, we …

Recent advances in far-field microscopy have demonstrated that fluorescence imaging is
possible at resolutions well below the long-standing diffraction limit. By exploiting …

Optical microscopy has been a cornerstone of life science investigations since its first
practical application around 400 years ago with the goal being subcellular resolution, three …

For almost a century, the resolution of optical microscopy was thought to be limited by
Abbé's law describing the diffraction limit of light. At the turn of the millennium, aided by new …

In this Perspective we propose our current point of view and a suggestive roadmap on the
field of high-resolution optical microscopy dedicated to bioimaging. Motivated by biological …

For more than a century, the resolution of focusing light microscopy has been limited by
diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently …

Fluorescent imaging microscopy has been an essential tool for biologists over many years,
especially after the discovery of the green fluorescent protein and the possibility of tagging …

The optical microscope is one of the oldest scientific instruments that is still used in forefront
research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let …

Until not very long ago, it was widely accepted that lens-based (far-field) optical microscopes
cannot visualize details much finer than about half the wavelength of light. The advent of …