[HTML][HTML] Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla

Y He, H Yan, W Hua, Y Huang, Z Wang - Frontiers in plant science, 2016 - frontiersin.org
Real time quantitative PCR (RT-qPCR or qPCR) has been extensively applied for analyzing
gene expression because of its accuracy, sensitivity, and high throughput. However, the …

Selection and validation of reference genes for quantitative RT-PCR analysis of gene expression in Populus trichocarpa

X Su, B Fan, L Yuan, X Cui, S Lu - Chinese Bulletin of Botany, 2013 - chinbullbotany.com
Quantitative RT-PCR (qRT-PCR) has been widely used in gene expression analysis
because of its sensitivity, specificity, and reproducibility. Application of suitable reference …

[HTML][HTML] Screening and Validation of Appropriate Reference Genes for Real-Time Quantitative PCR under PEG, NaCl and ZnSO4 Treatments in Broussonetia …

M Chen, Z Wang, Z Hao, H Li, Q Feng, X Yang… - International Journal of …, 2023 - mdpi.com
Real-time quantitative PCR (RT-qPCR) has a high sensitivity and strong specificity, and is
widely used in the analysis of gene expression. Selecting appropriate internal reference …

[HTML][HTML] Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time …

M Hu, W Hu, Z Xia, X Zhou, W Wang - Frontiers in Plant Science, 2016 - frontiersin.org
Reverse transcription quantitative real-time polymerase chain reaction (real-time PCR, also
referred to as quantitative RT-PCR or RT-qPCR) is a highly sensitive and high-throughput …

[HTML][HTML] Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

T Li, J Wang, M Lu, T Zhang, X Qu… - Frontiers in plant science, 2017 - frontiersin.org
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular
technique for investigating gene expression levels in plants. Based on the Minimum …

Evaluation of candidate reference genes for qPCR in maize

A Manoli, A Sturaro, S Trevisan, S Quaggiotti… - Journal of plant …, 2012 - Elsevier
Quantitative real-time PCR (qPCR) is a powerful tool to measure gene expression levels.
Accurate and reproducible results are dependent on the correct choice of the reference …

[HTML][HTML] Selection of reference genes for quantitative real-time PCR analysis in cucumber (Cucumis sativus L.), pumpkin (Cucurbita moschata Duch.) and cucumber …

L Miao, X Qin, L Gao, Q Li, S Li, C He, Y Li, X Yu - PeerJ, 2019 - peerj.com
Background Quantitative real-time PCR (qRT-PCR) is a commonly used high-throughput
technique to measure mRNA transcript levels. The accuracy of this evaluation of gene …

[HTML][HTML] Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Lycoris aurea

R Ma, S Xu, Y Zhao, B Xia, R Wang - Frontiers in plant science, 2016 - frontiersin.org
Lycoris aurea (L'Hér.) Herb, a perennial grass species, produces a unique variety of
pharmacologically active Amaryllidaceae alkaloids. However, the key enzymes and their …

[HTML][HTML] Evaluation of Reference Genes for Accurate Normalization of Gene Expression for Real Time-Quantitative PCR in Pyrus pyrifolia Using Different Tissue …

T Imai, BE Ubi, T Saito, T Moriguchi - PLoS One, 2014 - journals.plos.org
We have evaluated suitable reference genes for real time (RT)-quantitative PCR (qPCR)
analysis in Japanese pear (Pyrus pyrifolia). We tested most frequently used genes in the …

[HTML][HTML] Expression Stabilities of Ten Candidate Reference Genes for RT-qPCR in Zanthoxylum bungeanum Maxim

X Fei, Q Shi, T Yang, Z Fei, A Wei - Molecules, 2018 - mdpi.com
Real-time reverse transcription quantitative PCR has become a common method for
studying gene expression, however, the optimal selection of stable reference genes is a …