A HeLa cell 3'-exonuclease with properties of a mammalian mRNA poly (A) tail-removing
enzyme has been characterized. The exonuclease shows high specificity for the poly (A) tail …

We have previously identified a HeLa cell 3′ exonuclease specific for degrading poly (A)
tails of mRNAs. Here we report on the purification and identification of a calf thymus 54-kDa …

3′-Exonucleolytic removal of the poly (A) tail is the first and often rate-limiting step in the
decay of many eucaryotic mRNAs. In a cytoplasmic extract from HeLa cells, the poly (A) tail …

We have identified a 3′ exonuclease in HeLa cell extracts which deadenylates mammalian
mRNA and leaves the mRNA body intact after poly (A) removal. Only homopolymeric …

We have previously shown that the presence of a poly (A) tail blocks the activity of a highly
efficient 3'--> 5'exonuclease in HeLa extracts. Similar activities have been implicated in RNA …

We have developed an in vitro system which faithfully reproduces several aspects of general
mRNA stability. Poly (A)− RNAs were rapidly and efficiently degraded in this system with no …

Poly (A) tail removal is the first step in the degradation pathway for some mRNAs. The
purified poly (A)-binding protein (PAB)-dependent poly (A) nuclease (PAN) from yeast …

An exoribonuclease from calf thymus which specifically cleaves poly (A) in the single-or in
the double-stranded form has been isolated and purified to homogeneity. The enzyme has a …

Poly (A) tails of mRNA are nearly ubiquitous. In animal cells, the only mRNAs known to lack
poly (A) are those encoding the major histones (1-3). Poly (A) tails also occur in plants and …

Analysis of the slowed turnover rates of several specific mRNA species and the higher
cellular levels of some of these mRNAs in Saccharomyces cerevisiae lacking 5'→ …