The approach to sample acquisition and its impact on the derived human fecal microbiome and VOC metabolome

RD Couch, K Navarro, M Sikaroodi, P Gillevet… - PloS one, 2013 - journals.plos.org
Recent studies have illustrated the importance of the microbiota in maintaining a healthy
state, as well as promoting disease states. The intestinal microbiota exerts its effects …

Towards standards for human fecal sample preparation in targeted and untargeted lc-hrms studies

F Hosseinkhani, AC Dubbelman, N Karu, AC Harms… - Metabolites, 2021 - mdpi.com
Gut microbiota and their metabolic products are increasingly being recognized as important
modulators of human health. The fecal metabolome provides a functional readout of the …

Comparison of fecal collection methods on variation in gut metagenomics and untargeted metabolomics

H Guan, Y Pu, C Liu, T Lou, S Tan, M Kong, Z Sun… - Msphere, 2021 - Am Soc Microbiol
Integrative analysis of high-quality metagenomics and metabolomics data from fecal
samples provides novel clues for the mechanism underpinning gut microbe-human …

Coupling targeted and untargeted mass spectrometry for metabolome-microbiome-wide association studies of human fecal samples

AV Melnik, RR da Silva, ER Hyde, AA Aksenov… - Analytical …, 2017 - ACS Publications
Increasing appreciation of the gut microbiome's role in health motivates understanding the
molecular composition of human feces. To analyze such complex samples, we developed a …

Solid-phase microextraction and the human fecal VOC metabolome

E Dixon, C Clubb, S Pittman, L Ammann, Z Rasheed… - PloS one, 2011 - journals.plos.org
The diagnostic potential and health implications of volatile organic compounds (VOCs)
present in human feces has begun to receive considerable attention. Headspace solid …

Alcohol induced alterations to the human fecal VOC metabolome

RD Couch, A Dailey, F Zaidi, K Navarro, CB Forsyth… - PloS one, 2015 - journals.plos.org
Studies have shown that excessive alcohol consumption impacts the intestinal microbiota
composition, causing disruption of homeostasis (dysbiosis). However, this observed change …

Dual UHPLC-HRMS metabolomics and lipidomics and automated data processing workflow for comprehensive high-throughput gut phenotyping

P Vangeenderhuysen, J Van Arnhem… - Analytical …, 2023 - ACS Publications
In recent years, feces has surfaced as the matrix of choice for investigating the gut
microbiome-health axis because of its non-invasive sampling and the unique reflection it …

[PDF][PDF] Optimisation of sample preparation for direct SPME-GC-MS analysis of murine and human faecal volatile organic compounds for metabolomic studies

S Reade, A Mayor, R Aggio, T Khalid… - J Anal Bioanal …, 2014 - researchgate.net
The analysis of volatile organic compounds (VOCs) emitted from biological fluids such as
blood, urine, breath and faeces, has been receiving more attention from researchers and …

Strategy for Comprehensive Detection and Annotation of Gut Microbiota-Related Metabolites Based on Liquid Chromatography–High-Resolution Mass Spectrometry

S Zheng, W Qin, T Chen, R Ouyang, X Wang… - Analytical …, 2024 - ACS Publications
Gut microbiota, widely populating the mammalian gastrointestinal tract, plays an important
role in regulating diverse pathophysiological processes by producing bioactive molecules …

Evaluation of fresh, frozen, and lyophilized fecal samples by SPME and derivatization methods using GC× GC-TOFMS

SL Nam, K Tarazona Carrillo, AP de la Mata… - Metabolomics, 2022 - Springer
Introduction Feces is a highly complex matrix containing thousands of metabolites. It also
contains live bacteria and enzymes, and does not have a static chemistry. Consequently …