Deletion analysis of the Taka-amylase A gene promoter using a homologous transformation system in Aspergillus oryzae

K Tsuchiya, S Tada, K Gomi, K Kitamoto… - Bioscience …, 1992 - jstage.jst.go.jp
Materials and Methods Strains, plasmids, and media. A. oryzae M-2-3 (argB') was used as a
recipient strain for transformation.” Escherichia coli JM109 [rat/11,(lac-proAB), endAI …

Insertion analysis of putative functional elements in the promoter region of the Aspergillus oryzae Taka-amylase A gene (amyB) using a heterologous Aspergillus …

Y Kanemori, K Gomi, K Kitamoto… - Bioscience …, 1999 - jstage.jst.go.jp
抄録 Expression of the Taka-amylase A gene (amyB) of Aspergillus oryzae is induced by
starch or maltose. The A. oryzae amyB gene promoter contains three highly conserved …

Cloning and Nucleotide Sequence of the Genomic Taka-amylase A Gene of Aspergillus oryzae

S Tada, Y Iimura, K Gomi, K Takahashi… - Agricultural and …, 1989 - academic.oup.com
Abstract The Taka-amylase A (TAA) gene was cloned from the genomic library of A. oryzae
using synthetic DNA oligomers as probes. The gene was located in a 3.7 Kbp EcoRl …

Isolation of a cDNA encoding Aspergillus oryzae Taka-amylase A: evidence for multiple related genes

T Norihiro, F Makoto, N Hiroki, K Norie, T Akio… - Gene, 1989 - Elsevier
Complementary and genomic DNAs encoding Aspergillus oryzae Taka-amylase A (Taa)
were cloned and sequenced. The coding sequence of the cDNA comprised the signal …

Construction of a fusion gene comprising the Taka-amylase A promoter and the Escherichia coli β-glucuronidase gene and analysis of its expression in Aspergillus …

S Tada, K Gomi, K Kitamoto, K Takahashi… - Molecular and General …, 1991 - Springer
Northern blot analysis of glucose-grown and starch-grown mycelia of Aspergillus oryzae
R11340 was conducted using the cloned Taka-amylase A (TAA) gene as a probe. The …

Cloning of the α-amylase cDNA of Aspergillus shirousamii and its expression in Saccharomyces cerevisiae

I Shibuya, G Tamura, T Ishikawa… - Bioscience, biotechnology …, 1992 - jstage.jst.go.jp
Materials and Methods Strains, plasmids, and phages. A. shirousamii RIB2504 was used as
a donor strain to construct a cDNA library. E. coli strains HB101, 1 “JM109, 12'and …

[PDF][PDF] Overproduction of an α-Amylase/Glucoamylase Fusion Protein in Aspergillus oryzae Using a High Expression Vector

I Shibuya, K Tsuchiya, G Tamura… - Bioscience …, 1992 - Taylor & Francis
Ci-Amylase (AAase)[EC 3.2. 1.1, Ci-I, 4-glucan-4-glucanohydrolase] and glucoamylase
(GAase)[EC 3.2. 1.3, Ci-I, 4; I, 6-glucan glucohydrolase] are widely used in industrial …

Nucleotide Sequence and Expression of α-Glucosidase-encoding Gene (agdA) from Aspergillus oryzae

T Minetoki, K Gomi, K Kitamoto… - Bioscience …, 1995 - academic.oup.com
We have isolated an α-glucosidase (AGL)-encoding gene (agdA) from Aspergillus oryzae by
heterologous hybridization using the corresponding Aspergillus niger gene as a probe …

Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae …

T Minetoki, C Kumagai, K Gomi, K Kitamoto… - Applied microbiology …, 1998 - Springer
The role of the conserved sequence region III in the promoter regions of the amylase-
encoding genes amyB, glaA and agdA of Aspergillus oryzae was examined. Introduction of …

[PDF][PDF] Identification of the promoter region of the Taka-amylase A gene required for starch induction

S Tada, K Gomi, K Kitamoto, C Kumagai… - Agricultural and …, 1991 - Taylor & Francis
The original plasmid containing a fusion gene is shown in Fig. 1. This plasmid contains the
fusion of the promoter region of the TAA gene to the E. coli uidA structural gene previously …