Metabolic flux analysis of a phenol producing mutant of Pseudomonas putida S12: verification and complementation of hypotheses derived from transcriptomics

N Wierckx, HJ Ruijssenaars, JH de Winde… - Journal of …, 2009 - Elsevier
The physiological effects of genetic and transcriptional changes observed in a phenol
producing mutant of the solvent-tolerant Pseudomonas putida S12 were assessed with …

Transcriptome Analysis of a Phenol-Producing Pseudomonas putida S12 Construct: Genetic and Physiological Basis for Improved Production

NJP Wierckx, H Ballerstedt, JAM de Bont, JH de Winde… - 2008 - Am Soc Microbiol
The unknown genetic basis for improved phenol production by a recombinant
Pseudomonas putida S12 derivative bearing the tpl (tyrosine-phenol lyase) gene was …

Analysis of Pseudomonas putida growth on non‐trivial carbon sources using transcriptomics and genome‐scale modelling

I D'Arrigo, JGR Cardoso, M Rennig… - Environmental …, 2019 - Wiley Online Library
Pseudomonas putida is characterized by a versatile metabolism and stress tolerance traits
that allow the bacterium to cope with different environmental conditions. In this work, the …

Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons in Pseudomonas putida

MA Kukurugya, CM Mendonca, M Solhtalab… - Journal of Biological …, 2019 - ASBMB
Pseudomonas species thrive in different nutritional environments and can catabolize
divergent carbon substrates. These capabilities have important implications for the role of …

Metabolic engineering of Pseudomonas taiwanensis VLB120 with minimal genomic modifications for high-yield phenol production

B Wynands, C Lenzen, M Otto, F Koch, LM Blank… - Metabolic …, 2018 - Elsevier
Aromatic chemicals are important building blocks for the production of a multitude of
everyday commodities. Currently, aromatics production relies almost exclusively on …

Convergent Peripheral Pathways Catalyze Initial Glucose Catabolism in Pseudomonas putida: Genomic and Flux Analysis

T Del Castillo, JL Ramos… - Journal of …, 2007 - Am Soc Microbiol
In this study, we show that glucose catabolism in Pseudomonas putida occurs through the
simultaneous operation of three pathways that converge at the level of 6-phosphogluconate …

Functional implementation of a linear glycolysis for sugar catabolism in Pseudomonas putida

A Sánchez-Pascuala, L Fernández-Cabezón… - Metabolic …, 2019 - Elsevier
The core metabolism for glucose assimilation of the soil bacterium and platform strain
Pseudomonas putida KT2440 has been reshaped from the native, cyclically-operating …

Comprehensive analysis of the metabolome of Pseudomonas putida S12 grown on different carbon sources

MJ van der Werf, KM Overkamp, B Muilwijk… - Molecular …, 2008 - pubs.rsc.org
Metabolomics is an emerging, powerful, functional genomics technology that involves the
comparative non-targeted analysis of the complete set of metabolites in an organism. We …

Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol

V Beckers, I Poblete-Castro, J Tomasch… - Microbial Cell …, 2016 - Springer
Background Given its high surplus and low cost, glycerol has emerged as interesting carbon
substrate for the synthesis of value-added chemicals. The soil bacterium Pseudomonas …

A cyclic metabolic network in Pseudomonas protegens Pf-5 prioritizes the Entner-Doudoroff pathway and exhibits substrate hierarchy during carbohydrate co …

RA Wilkes, CM Mendonca… - Applied and environmental …, 2019 - Am Soc Microbiol
The genetic characterization of Pseudomonas protegens Pf-5 was recently completed.
However, the inferred metabolic network structure has not yet been evaluated …