Raman spectroscopy detects biochemical changes due to different cell culture environments in live cells in vitro

M Gargotti, E Efeoglu, HJ Byrne, A Casey - Analytical and bioanalytical …, 2018 - Springer
The in vitro cell culture environment can impact on cell biochemistry and cell cycle. The
manifestation of such substrate-induced changes in cell cycle in the Raman …

Three dimensional collagen gels as a cell culture matrix for the study of live cells by Raman spectroscopy

F Bonnier, AD Meade, S Merzha, P Knief… - Analyst, 2010 - pubs.rsc.org
Three dimensional collagen gels are evaluated as matrices for the study of live cells by
Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and …

Monitoring cellular behaviour using Raman spectroscopy for tissue engineering and regenerative medicine applications

AR Boyd, GA Burke, BJ Meenan - Journal of Materials Science: Materials …, 2010 - Springer
Raman spectroscopy has been used to determine the chemical composition of materials for
over 70 years. Recent spectacular advances in laser and CCD camera technology creating …

Collagen matrices as an improved model for in vitro study of live cells using Raman microspectroscopy

F Bonnier, P Knief, AD Meade, J Dorney… - … on Biomedical Optics, 2011 - opg.optica.org
Due to its high lateral resolution, Raman microspectrsocopy is rapidly becoming an
accepted technique for the subcellular imaging of single cells. Although the potential of the …

Non‐invasive analysis of cell cycle dynamics in single living cells with Raman micro‐spectroscopy

RJ Swain, G Jell, MM Stevens - Journal of cellular biochemistry, 2008 - Wiley Online Library
Raman micro‐spectroscopy is a laser‐based technique which enables rapid and non‐
invasive biochemical analysis of cells and tissues without the need for labels, markers or …

Imaging live cells grown on a three dimensional collagen matrix using Raman microspectroscopy

F Bonnier, P Knief, B Lim, AD Meade, J Dorney… - Analyst, 2010 - pubs.rsc.org
Three dimensional collagen gels have been used as matrices for the imaging of live cells by
Raman spectroscopy. The study is conducted on a human lung adenocarcinoma (A549) and …

Raman spectroscopy in biomedicine–non‐invasive in vitro analysis of cells and extracellular matrix components in tissues

E Brauchle, K Schenke‐Layland - Biotechnology journal, 2013 - Wiley Online Library
Raman spectroscopy is an established laser‐based technology for the quality assurance of
pharmaceutical products. Over the past few years, Raman spectroscopy has become a …

In situ characterisation of living cells by Raman spectroscopy

I Notingher, S Verrier, H Romanska, AE Bishop… - …, 2002 - content.iospress.com
We report the first Raman spectra of individual living and dead cells (MLE‐12 line) cultured
on bioinert standard poly‐L‐lysine coated fused silica and on bioactive 45S5 Bioglass® …

Progress in Raman spectroscopy in the fields of tissue engineering, diagnostics and toxicological testing

CA Owen, I Notingher, R Hill, M Stevens… - Journal of Materials …, 2006 - Springer
This review summarises progress in Raman spectroscopy and its application in diagnostics,
toxicological testing and tissue engineering. Applications of Raman spectroscopy in cell …

Variability in Raman Spectra of Single Human Tumor Cells Cultured in Vitro: Correlation with Cell Cycle and Culture Confluency

Q Matthews, A Jirasek, J Lum, X Duan… - Applied …, 2010 - opg.optica.org
In this work we investigate the capability of Raman microscopy (RM) to detect inherent
sources of biochemically based spectral variability between single cells of a human tumor …