Harmonic generation microscopy 2.0: new tricks empowering intravital imaging for neuroscience
H Lim - Frontiers in Molecular Biosciences, 2019 - frontiersin.org
Optical harmonic generation, eg, second-(SHG) and third-harmonic generation (THG),
provides intrinsic contrasts for three-dimensional intravital microscopy. Contrary to two …
provides intrinsic contrasts for three-dimensional intravital microscopy. Contrary to two …
High throughput second harmonic imaging for label-free biological applications
C Macias-Romero, MEP Didier, P Jourdain… - Optics express, 2014 - opg.optica.org
Second harmonic generation (SHG) is inherently sensitive to the absence of spatial
centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical …
centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical …
Label-free live brain imaging and targeted patching with third-harmonic generation microscopy
The ability to visualize neurons inside living brain tissue is a fundamental requirement in
neuroscience and neurosurgery. Especially the development of a noninvasive probe of …
neuroscience and neurosurgery. Especially the development of a noninvasive probe of …
Second-and third-harmonic generation microscopies for the structural imaging of intact tissues
One principal advantage of multiphoton excitation microscopy is that it preserves its three-
dimensional micrometer resolution when imaging inside light-scattering samples. For that …
dimensional micrometer resolution when imaging inside light-scattering samples. For that …
Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues
PJ Campagnola, AC Millard, M Terasaki, PE Hoppe… - Biophysical journal, 2002 - cell.com
We find that several key endogenous protein structures give rise to intense second-
harmonic generation (SHG)—nonabsorptive frequency doubling of an excitation laser line …
harmonic generation (SHG)—nonabsorptive frequency doubling of an excitation laser line …
Multimodal two-photon imaging using a second harmonic generation-specific dye
M Nuriya, S Fukushima, A Momotake… - Nature …, 2016 - nature.com
Second harmonic generation (SHG) imaging can be used to visualize unique biological
phenomena, but currently available dyes limit its application owing to the strong fluorescent …
phenomena, but currently available dyes limit its application owing to the strong fluorescent …
[HTML][HTML] Recent advancements in optical harmonic generation microscopy: Applications and perspectives
S JamesDarian, J CampagnolaPaul - BME frontiers, 2021 - spj.science.org
Second harmonic generation (SHG) and third harmonic generation (THG) microscopies
have emerged as powerful imaging modalities to examine structural properties of a wide …
have emerged as powerful imaging modalities to examine structural properties of a wide …
Three‐photon‐resonance‐enhanced third‐harmonic generation for label‐free deep‐brain imaging: In search of a chemical contrast
AA Lanin, AS Chebotarev… - Journal of Raman …, 2019 - Wiley Online Library
Within the past decade, nonlinear Raman microscopy has earned a well‐deserved status of
a gold‐standard technology for chemically selective imaging. Even though second‐and third …
a gold‐standard technology for chemically selective imaging. Even though second‐and third …
Dyes for biological second harmonic generation imaging
Nonlinear optical imaging has revolutionized microscopy for the life sciences. Second
harmonic generation (SHG), the younger sibling of two-photon excited fluorescence (2PF), is …
harmonic generation (SHG), the younger sibling of two-photon excited fluorescence (2PF), is …
Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window
Y Murakami, M Masaki, S Miyazaki… - Biomedical Optics …, 2022 - opg.optica.org
In this study, second harmonic generation (SHG) and third harmonic generation (THG)
spectroscopic imaging were performed on biological samples using a femtosecond laser …
spectroscopic imaging were performed on biological samples using a femtosecond laser …