CRISPR-Cas9 and related technologies enable the efficient alteration of genomic DNA at
targeted positions and are widely used in applications ranging from individual gene …

The CRISPR-Cas9 system has successfully been adapted to edit the genome of various
organisms. However, our ability to predict the editing outcome at specific sites is limited …

CRISPR-Cas9 technology allows the creation of user-defined genomic modifications in cells
and whole organisms. However, quantifying editing rates in pools of cells or identifying …

CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the
functionality of genetic elements, creating genetically modified organisms as well as …

As the possibilities of CRISPR-Cas9 technology have been revealed, we have entered a
new era of research aimed at increasing its specificity and safety. This stage of technology …

Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics.
The ease of this technique has enabled genome engineering in-vitro and in a range of …

CRISPR-based approaches have quickly become a favored method to perturb genes to
uncover their functions. Here, we review the key considerations in the design of genome …

Advances in the development of delivery, repair, and specificity strategies for the CRISPR-
Cas9 genome engineering toolbox are helping researchers understand gene function with …

Over the past decade, CRISPR/Cas-based gene editing has become a powerful tool for
generating mutations in a variety of model organisms, from Escherichia coli to zebrafish …

CRISPR/Cas9 technology has evolved as the most powerful approach to generate genetic
models both for fundamental and preclinical research. Despite its apparent simplicity, the …