Development and evaluation of a rapid and sensitive EBOV-RPA test for rapid diagnosis of Ebola virus disease

M Yang, Y Ke, X Wang, H Ren, W Liu, H Lu, W Zhang… - Scientific reports, 2016 - nature.com
Confirming Ebola virus disease (EVD), a deadly infectious disease, requires real-time RT-
PCR, which takes up to a few hours to yield results. Therefore, a rapid diagnostic assay is …

Evaluation of a rapid and sensitive RT-qPCR assay for the detection of Ebola Virus

M Biava, F Colavita, A Marzorati, D Russo… - Journal of virological …, 2018 - Elsevier
Abstract Background The 2013–2016 Ebola virus disease (EVD) outbreak showed a lack of
diagnostic point-of-care methods. Currently, EBOV diagnosis relies on quantitative reverse …

Development, evaluation, and integration of a quantitative reverse-transcription polymerase chain reaction diagnostic test for Ebola virus on a molecular diagnostics …

L Cnops, P Van den Eede, J Pettitt… - The Journal of …, 2016 - academic.oup.com
Abstract Background. The 2013–2016 Ebola epidemic in West Africa resulted in accelerated
development of rapid diagnostic tests for emergency outbreak preparedness. We describe …

Field validation of the ReEBOV antigen rapid test for point-of-care diagnosis of Ebola virus infection

ML Boisen, RW Cross, JN Hartnett… - The Journal of …, 2016 - academic.oup.com
Abstract Background. The 2013–2016 West African Ebola virus disease (EVD) epidemic is
the largest recorded. Triage on the basis of clinical signs had limited success, and the time …

ReEBOV Antigen Rapid Test kit for point-of-care and laboratory-based testing for Ebola virus disease: a field validation study

MJ Broadhurst, JD Kelly, A Miller, A Semper, D Bailey… - The Lancet, 2015 - thelancet.com
Background At present, diagnosis of Ebola virus disease requires transport of venepuncture
blood to field biocontainment laboratories for testing by real-time RT-PCR, resulting in …

Analytical validation of the ReEBOV antigen rapid test for point-of-care diagnosis of Ebola virus infection

RW Cross, ML Boisen, MM Millett… - The Journal of …, 2016 - academic.oup.com
Background. Ebola virus disease (EVD) is a severe viral illness caused by Ebola virus
(EBOV). The 2013–2016 EVD outbreak in West Africa is the largest recorded, with> 11 000 …

Analytical performance characteristics of the Cepheid GeneXpert Ebola assay for the detection of Ebola virus

BA Pinsky, MK Sahoo, J Sandlund, M Kleman… - PloS one, 2015 - journals.plos.org
Background The recently developed Xpert® Ebola Assay is a novel nucleic acid
amplification test for simplified detection of Ebola virus (EBOV) in whole blood and buccal …

Evaluation of the Biofire FilmArray BioThreat-E Test (v2. 5) for rapid identification of ebola virus disease in heat-treated blood samples obtained in Sierra Leone and …

SA Weller, D Bailey, S Matthews… - Journal of clinical …, 2016 - Am Soc Microbiol
Rapid Ebola virus (EBOV) detection is crucial for appropriate patient management and care.
The performance of the FilmArray BioThreat-E test (v2. 5) using whole-blood samples was …

Performance of the GeneXpert Ebola assay for diagnosis of Ebola virus disease in Sierra Leone: a field evaluation study

AE Semper, MJ Broadhurst, J Richards… - PLoS …, 2016 - journals.plos.org
Background Throughout the Ebola virus disease (EVD) epidemic in West Africa, field
laboratory testing for EVD has relied on complex, multi-step real-time reverse transcription …

Interpretation of negative molecular test results in patients with suspected or confirmed Ebola virus disease: report of two cases

JK Edwards, C Kleine, V Munster… - Open forum …, 2015 - academic.oup.com
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is the most
sensitive quantitative diagnostic assay for detection of Ebola virus in multiple body fluids …