CRISPR-Cas9 mediated mutagenesis of a DMR6 ortholog in tomato confers broad-spectrum disease resistance

D Paula de Toledo Thomazella, Q Brail, D Dahlbeck… - BioRxiv, 2016 - biorxiv.org
Pathogenic microbes are responsible for severe production losses in crops worldwide. The
use of disease resistant crop varieties can be a sustainable approach to meet the food …

[HTML][HTML] Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

V Nekrasov, C Wang, J Win, C Lanz, D Weigel… - Scientific reports, 2017 - nature.com
Genome editing has emerged as a technology with a potential to revolutionize plant
breeding. In this study, we report on generating, in less than ten months, Tomelo, a non …

Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance

DPT Thomazella, K Seong… - Proceedings of the …, 2021 - National Acad Sciences
Plant diseases are among the major causes of crop yield losses around the world. To confer
disease resistance, conventional breeding relies on the deployment of single resistance (R) …

[HTML][HTML] Plant and fungal genome editing to enhance plant disease resistance using the CRISPR/Cas9 system

NC Paul, SW Park, H Liu, S Choi, J Ma… - Frontiers in Plant …, 2021 - frontiersin.org
Crop production has been substantially reduced by devastating fungal and oomycete
pathogens, and these pathogens continue to threaten global food security. Although …

CRISPR crops: plant genome editing toward disease resistance

T Langner, S Kamoun, K Belhaj - Annual review of …, 2018 - annualreviews.org
Genome editing by sequence-specific nucleases (SSNs) has revolutionized biology by
enabling targeted modifications of genomes. Although routine plant genome editing …

Genome editing: targeting susceptibility genes for plant disease resistance

SSA Zaidi, MS Mukhtar, S Mansoor - Trends in Biotechnology, 2018 - cell.com
Plant pathogens pose a major threat to crop productivity. Typically, phytopathogens exploit
plants' susceptibility (S) genes to facilitate their proliferation. Disrupting these S genes may …

[PDF][PDF] Precision breeding made real with CRISPR: illustration through genetic resistance to pathogens

F Veillet, M Durand, T Kroj, S Cesari, JL Gallois - Plant Communications, 2020 - cell.com
Since its discovery as a bacterial adaptive immune system and its development for genome
editing in eukaryotes, the CRISPR technology has revolutionized plant research and …

[HTML][HTML] CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

MI Santillán Martínez, V Bracuto, E Koseoglou… - BMC plant biology, 2020 - Springer
Background The development of CRISPR/Cas9 technology has facilitated targeted
mutagenesis in an efficient and precise way. Previously, RNAi silencing of the susceptibility …

[HTML][HTML] Genome engineering technology for durable disease resistance: Recent progress and future outlooks for sustainable agriculture

Q Ali, C Yu, A Hussain, M Ali, S Ahmar… - Frontiers in Plant …, 2022 - frontiersin.org
Crop production worldwide is under pressure from multiple factors, including reductions in
available arable land and sources of water, along with the emergence of new pathogens …

[HTML][HTML] DNA-free genetically edited grapevine and apple protoplast using CRISPR/Cas9 ribonucleoproteins

M Malnoy, R Viola, MH Jung, OJ Koo, S Kim… - Frontiers in plant …, 2016 - frontiersin.org
The combined availability of whole genome sequences and genome editing tools is set to
revolutionize the field of fruit biotechnology by enabling the introduction of targeted genetic …