Luciferase is a popular enzyme used for biological analyses, such as reporter assays. In
addition to a conventional reporter assay using a pair of firefly and Renilla luciferases, a …

The bright bioluminescence of copepod Metridia longa is conditioned by a small secreted
coelenterazine-dependent luciferase (MLuc). To date, three isoforms of MLuc differing in …

The technology of real-time imaging in living cells is crucial for understanding of intracellular
events. For this purpose, bioluminescent reporters have been introduced as sensitive and …

Due to its ability to emit light, the luciferase from Renilla reniformis (RLuc) is widely
employed in molecular biology as a reporter gene in cell culture experiments and small …

This study elucidates functional artificial luciferases (ALucs) wholly synthesized for
bioassays and molecular imaging. The ALucs bearing epitopes were newly created by …

A luciferase from the railroad worm (Phrixothrix hirtus) is the only red‐emitting
bioluminescent enzyme in nature that is advantageous in multicolor luciferase assays and in …

The recombinant coelenterazine-dependent luciferases (isoforms MLuc164 and MLuc39)
from the marine copepod Metridia longa were expressed asinclusion bodies in E. coli cells …

Blue fluorescent protein from the calcium-sensitive photoprotein aequorin (BFP-aq) was
prepared and determined to be a heat resistant enzyme, catalyzing the luminescent …

The present study demonstrates cation-driven optical properties of artificial luciferases
(ALucs) from copepod luciferases, as an optical readout for bioanalysis. An assignment of …

Significant amino acid sequence homology in two regions of Vargula hilgendorfii to one in
apoaequorin was reported. The intra-amino acid homology in Vargula luciferase between …