Background: Metabolomics aims to characterize the metabolic phenotype and metabolic
pathways utilized by microorganisms or other cellular systems. A crucial component to …

Pseudomonas aeruginosa is a common, Gram-negative environmental organism. It can be a
significant pathogenic factor of severe infections in humans, especially in cystic fibrosis …

Obtaining meaningful snapshots of the metabolome of microorganisms requires rapid
sampling and immediate quenching of all metabolic activity, to prevent any changes in …

We grew Pseudomonas aeruginosa in LB and artificial sputum medium (ASM)(filtered and
unfiltered) and quantified metabolite utilization and excretion by nuclear magnetic …

In addition to transcriptome and proteome studies, metabolome analysis represents a third
complementary approach to identify metabolic pathways and adaptation processes. In order …

Microorganisms produce and secrete many primary and secondary metabolites to the
surrounding environment during their growth. Therefore, extracellular metabolites provide …

The P seudomonas a eruginosa M etabolome D ata b ase (PAMDB, http://pseudomonas.
umaryland. edu) is a searchable, richly annotated metabolite database specific to P …

Absolute metabolite concentrations are essential information for quantitative metabolomics
studies, as concentrations are closely related to metabolic reactions, enzyme kinetics and …

Understanding metabolism is fundamental to access and harness bacterial physiology. In
most bacteria, nutrient utilization is hierarchically optimized according to their energetic …

Uptake and excretion of nutrients is an integral part of a cell's physiology. Using analytical
chemistry techniques, metabolite uptake and excretion from the culture medium can be …