On display: autotransporter secretion and application

P Van Ulsen, KM Zinner, WSP Jong… - FEMS microbiology …, 2018 - academic.oup.com
The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria
to export virulence and colonization factors to their cell surface or into their surroundings …

[HTML][HTML] Autotransporter secretion: varying on a theme

J Grijpstra, J Arenas, L Rutten, J Tommassen - Research in microbiology, 2013 - Elsevier
Autotransporters are widely distributed among Gram-negative bacteria. They can have a
large variety of functions and many of them have a role in virulence. They are synthesized as …

Overproducing the BAM complex improves secretion of difficult-to-secrete recombinant autotransporter chimeras

TH Phan, C Kuijl, DT Huynh, WSP Jong, J Luirink… - Microbial Cell …, 2021 - Springer
Monomeric autotransporters have been used extensively to transport recombinant proteins
or protein domains to the cell surface of Gram-negative bacteria amongst others for antigen …

Comparing autotransporter β-domain configurations for their capacity to secrete heterologous proteins to the cell surface

WSP Jong, M Schillemans, CM ten Hagen-Jongman… - PLoS …, 2018 - journals.plos.org
Monomeric autotransporters have been extensively used for export of recombinant proteins
to the cell surface of Gram-negative bacteria. A bottleneck in the biosynthesis of such …

Autotransporter-based cell surface display in Gram-negative bacteria

T Nicolay, J Vanderleyden… - Critical reviews in …, 2015 - Taylor & Francis
Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

A structurally informed autotransporter platform for efficient heterologous protein secretion and display

WSP Jong, Z Soprova, K de Punder… - Microbial cell …, 2012 - Springer
Background The self-sufficient autotransporter (AT) pathway, ubiquitous in Gram-negative
bacteria, combines a relatively simple protein secretion mechanism with a high transport …

Protein secretion in gram-negative bacteria via the autotransporter pathway

N Dautin, HD Bernstein - Annu. Rev. Microbiol., 2007 - annualreviews.org
Autotransporters are a large and diverse superfamily of proteins produced by pathogenic
gram-negative bacteria that are composed of an N-terminal passenger domain, which …

BamA is required for autotransporter secretion

D Ryoo, MO Rydmark, YT Pang, KP Lundquist… - … et Biophysica Acta (BBA …, 2020 - Elsevier
Abstract Background In Gram-negative bacteria, type Va and Vc autotransporters are
proteins that contain both a secreted virulence factor (the “passenger” domain) and a β …

From self sufficiency to dependence: mechanisms and factors important for autotransporter biogenesis

DL Leyton, AE Rossiter, IR Henderson - Nature Reviews Microbiology, 2012 - nature.com
Autotransporters are a superfamily of proteins that use the type V secretion pathway for their
delivery to the surface of Gram-negative bacteria. At first glance, autotransporters look to …

Surface display of proteins by Gram-negative bacterial autotransporters

N Rutherford, M Mourez - Microbial cell factories, 2006 - Springer
Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …