Development of in-vitro-derived bovine embryos in protein-free media: effects of amino acids, glucose, pyruvate, lactate, phosphate and osmotic pressure

G Wirtu, CE Pope, P Damiani, F Miller… - Reproduction …, 2004 - CSIRO Publishing
In experiment 1, the effects of a group of either 20 (ie glutamine+ essential+ non-essential)
or 11 (ie hamster embryo culture medium (HECM)-6) amino acids were evaluated in …

In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, amino acids and vitamins

Y Takahashi, NL First - Theriogenology, 1992 - Elsevier
To elucidate the effect of nutrient substrates on embryo development, in vitro fertilized
bovine one-cell embryos were cultured in a medium similar to synthetic oviduct fluid (SOF) …

Effects of amino acids on development in vitro of cleavage-stage bovine embryos into blastocysts

T Pinyopummintr, BD Bavister - Reproduction, fertility and …, 1996 - CSIRO Publishing
Effects of amino acids on early bovine embryo development in vitro were examined using a
chemically-defined, protein-free culture medium. Bovine embryos produced in vitro were …

Effects of fructose supplementation in chemically defined protein-free medium on development of bovine in vitro fertilized embryos

MMU Bhuiyan, SK Kang, BC Lee - Animal reproduction science, 2007 - Elsevier
The present study evaluated the possible embryotrophic role of fructose supplementation in
potassium simplex optimization medium (KSOM) on preimplantation development of bovine …

Consumption of amino acids by in vivo and in vitro-derived bovine embryos

RJ Partridge, HJ Leese, D Pullar, AE Wrathall - Theriogenology, 1996 - infona.pl
Amino acids have been shown to improve the development of in vitro fertilised bovine 1-cell
embryos to morulae and blastocysts, and increase total cell numbers at the blastocyst stage …

Effect of exogenous carbohydrates in a serum-free culture medium on the development of in vitro matured and fertilized porcine embryos

Y Park, J Hong, H Yong, J Lim, E Lee - Zygote, 2005 - cambridge.org
This study was conducted to examine the effect of energy substrates in a serum-free culture
medium on in vitro development of porcine embryos. Presumptive zygotes derived from in …

Macromolecular source as dependent on osmotic pressure and water source: effects on bovine in vitro embryo development and quality

P Duque, CO Hidalgo, E Gómez… - Reproduction …, 2003 - rnd.edpsciences.org
This study evaluated the protective effect of protein, as dependent on osmolarity, and the
quality of water sources used to prepare embryo culture media. In Experiment 1, two …

[HTML][HTML] Less is more: Reduced nutrient concentration during in vitro culture improves embryo production rates and morphophysiology of bovine embryos

EC Dos Santos, AM da Fonseca Junior, CB de Lima… - Theriogenology, 2021 - Elsevier
Reproducing the environment to which the embryo is naturally exposed may be an
alternative to improve viability of embryos produced in vitro. In the first part of this work, we …

Energy and protein sources for development of pig embryos cultured beyond hatching in vitro

BA Stone, P Quinn, RF Seamark - Animal Reproduction Science, 1984 - Elsevier
The effects of supplementation of synthetic culture media with different energy and protein
sources on in vitro development of pig embryos beyond the 4–8-cell stage have been …

Glucose requirement at different developmental stages of in vitro fertilized bovine embryos cultured in semi-defined medium

JH Kim, H Funahashi, K Niwa, K Okuda - Theriogenology, 1993 - Elsevier
Three experiments were conducted in which 2-cell bovine embryos were prepared from
oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by …