Energy and protein sources for development of pig embryos cultured beyond hatching in vitro

BA Stone, P Quinn, RF Seamark - Animal Reproduction Science, 1984 - Elsevier
The effects of supplementation of synthetic culture media with different energy and protein
sources on in vitro development of pig embryos beyond the 4–8-cell stage have been …

Interaction between embryos and culture conditions during in vitro development of bovine early embryos

Y Nagao, R Iijima, K Saeki - Zygote, 2008 - cambridge.org
Various factors such as embryo density and substances in the medium can influence
embryo development in vitro. These factors and the embryos probably interact with each …

The Effect of Energy Substrate Concentration and Amino Acids on the In Vitro Development of Preimplantation Porcine Embryos

LFS Beebe, S McIlfactrick, MB Nottle - Cloning and Stem Cells, 2007 - liebertpub.com
As the pig becomes increasingly used for biomedical research, an effective and efficient in
vitro culture system is essential. This study aimed to improve the commonly used porcine …

Requirement for glucose during in vitro culture of sheep preimplantation embryos

JG Thompson, AC Simpson, PA Pugh… - Molecular …, 1992 - Wiley Online Library
Glucose utilization by sheep embryos was examined in 8‐cell (N= 36) and blastocyst (N=
36) stages, by measuring conversion of [5− 3H] glucose to 3H2O. Fifty percent glucose …

[HTML][HTML] Developmental and molecular response of bovine embryos to reduced nutrients in vitro

JR Herrick, S Rajput, R Pasquariello… - Reproduction and …, 2020 - raf.bioscientifica.com
Recent studies in our laboratory have indicated that bovine embryos only use a small
amount of the nutrients available to them in culture. Our objective was to evaluate the …

Effects of amino acids on the development of in-vitro matured/in-vitro fertilization bovine embryos in a simple protein-free medium

Z Liu, RH Foote - Human Reproduction, 1995 - academic.oup.com
The effects of commercially available amino acids, referred to as Eagle's non-essential
amino acids (NEAA) and essential amino acids (EAA), on the development of bovine …

Supplementation of fructose in chemically defined protein-free medium enhances the in vitro development of bovine transgenic cloned embryos

MMU Bhuiyan, SK Kang, BC Lee - Zygote, 2007 - cambridge.org
The present study evaluated the possible embryotrophic role of fructose supplementation in
chemically defined protein-free KSOM on in vitro development of bovine transgenic cloned …

Glucose and glycine synergistically enhance the in vitro development of porcine blastocysts in a chemically defined medium

T Mito, K Yoshioka, S Yamashita… - Reproduction …, 2012 - CSIRO Publishing
In the present study, the effects of glucose and/or glycine on the in vitro development of Day
5 (Day 0= IVF) porcine blastocysts were determined. The addition of 2.5–10 mM glucose to …

Development of pig blastocysts in vitro is altered by serum, bovine serum albumin and amino acids and vitamins

BA Meyen, CF Rosenkrans Jr, DL Davis - Theriogenology, 1989 - Elsevier
We tested the effects of the amino acids and vitamins in minimum essential medium (MEM)
and Eagle's medium (BME) on pig blastocyst development and nuclei number. Embryos …

Studies on a chemically defined medium for in vitro culture of in vitro matured and fertilized porcine oocytes

T Iwasaki, E Kimura, K Totsukawa - Theriogenology, 1999 - Elsevier
The present study evaluated the effects of various components in a chemically defined
medium on the development of IVM/IVF porcine embryos. The investigated components …