Although toxic for early stages of embryo development, glucose is a physiological metabolic
substrate at the morula and blastocyst stages. We evaluated the effect of adding 5.5 mM …

Bovine oocytes that had been matured and fertilized in vitro were cultured in a simple,
chemically defined, protein-free medium (mTLP-PVA). When the medium was supplemented …

Three experiments investigated the effects of culture systems and renewal of medium
(synthetic oviduct fluid medium (SOFM) supplemented with glutamine and amino acids) …

The aims of this study were to investigate improvements to the pig preimplantation embryo
culture system using in vitro produced embryos. For experiment 1, the optimum time to …

The effect of glycine and alanine on the development of 2-to 4-cell bovine embryos, and
amino acid uptake by bovine morulae and blastocysts, were examined through the use of a …

Simple Summary Supplementation of the culture media for the in vitro production of bovine
embryos with fetal bovine serum (FBS) is associated with inconsistent outcomes. This study …

The aim of this study was to develop a serum-free culture system that could support high
levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this …

The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …

The effect of glucose supplementation at different times in in vitro culture on the
developmental competence of in vitro produced (IVP) porcine embryos was examined. In …

The effects of glutamine, hypotaurine, taurine and premixed solutions of essential amino
acids (EAA) and non-essential amino acids (NEAA) on in vitro development of porcine …