Quantitative time-lapse imaging data of single cells expressing the transmembrane protein,
vesicular stomatitis virus ts045 G protein fused to green fluorescent protein (VSVG–GFP) …

The mechanisms and carriers responsible for exocytic protein trafficking between the trans-
Golgi network (TGN) and the plasma membrane remain unclear. To investigate the …

Biosynthetic transport from the trans-Golgi network (TGN) to the plasma membrane (PM) is
mediated by secretory vesicles. We analyzed secretory vesicle transport in real time using a …

Constitutive secretion is used to deliver newly synthesized proteins to the cell surface and to
the extracellular milieu. The trans-Golgi network is a key station along this route that …

Secretory proteins enter the Golgi apparatus when transport vesicles fuse with the cis-side
and exit in transport vesicles budding from the trans-side. Resident Golgi enzymes that have …

Recent studies using GFP-tagged markers and time-lapse microscopy have allowed direct
visualisation of membrane traffic in the secretory pathway in living mammalian cells. This …

The role of vesicles in cargo transport through the Golgi apparatus has been controversial.
Large forms of cargo such as protein aggregates are thought to progress through the Golgi …

Transport of constitutive cargo proteins from the Golgi complex to the plasma membrane
(PM) is known to be mediated by large tubular-saccular carriers moving along microtubules …

To describe trafficking of secretory cargo within the Golgi apparatus, the cisternal maturation
model predicts that Golgi cisternae change their properties from cis to trans while cargo …

The sorting of secreted cargo proteins and their export from the trans-Golgi network (TGN)
remains an enigma in the field of membrane trafficking; although the sorting mechanisms of …