We describe a chemical proteomics approach to profile the interaction of small molecules
with hundreds of endogenously expressed protein kinases and purine-binding proteins. This …

Solid supported probes have proven to be an efficient tool for chemical proteomics. The
kinobeads technology features kinase inhibitors covalently attached to Sepharose for affinity …

Protein kinases (PKs) play an important role in the development and progression of cancer
by regulating cell growth, survival, invasion, metastasis, and angiogenesis. Dasatinib (BMS …

Kinobeads are a set of promiscuous kinase inhibitors immobilized on sepharose beads for
the comprehensive enrichment of endogenously expressed protein kinases from cell lines …

Medicinal chemistry has discovered thousands of potent protein and lipid kinase inhibitors.
These may be developed into therapeutic drugs or chemical probes to study kinase biology …

Protein kinases comprise a large family of structurally related enzymes. A major goal in
kinase-inhibitor development is to selectively engage the desired kinase while avoiding …

INTRODUCTION Molecularly targeted drugs such as imatinib and crizotinib have
revolutionized the treatment of certain blood and lung cancers because of their remarkable …

Herein we describe three applications of label-free kinase profiling using a novel type of
phosphate affinity polyacrylamide gel electrophoresis. The phosphate affinity site is a …

Eukaryotic and prokaryotic organisms possess huge numbers of uncharacterized enzymes.
Selective inhibitors offer powerful probes for assigning functions to enzymes in native …

The medical and pharmaceutical communities are facing a dire need for new druggable
targets, while, paradoxically, the targets of some drugs that are in clinical use or …