[HTML][HTML] Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat
H Hamada, Y Liu, Y Nagira, R Miki, N Taoka, R Imai - Scientific reports, 2018 - nature.com
The current application of genome editing to crop plants is limited to cultivars that are
amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing …
amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing …
Development of an Agrobacterium‐delivered CRISPR/Cas9 system for wheat genome editing
CRISPR/Cas9 has been widely used for genome editing in many organisms, including
important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the …
important crops like wheat. Despite the tractability in designing CRISPR/Cas9, efficacy in the …
[HTML][HTML] Bidirectional promoter-based CRISPR-Cas9 systems for plant genome editing
Q Ren, Z Zhong, Y Wang, Q You, Q Li, M Yuan… - Frontiers in plant …, 2019 - frontiersin.org
CRISPR-Cas systems can be expressed in multiple ways, with different capabilities
regarding tissue-specific expression, efficiency, and expression levels. Thus far, three …
regarding tissue-specific expression, efficiency, and expression levels. Thus far, three …
[HTML][HTML] Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering
N Wada, R Ueta, Y Osakabe, K Osakabe - BMC Plant Biology, 2020 - Springer
Traditionally, generation of new plants with improved or desirable features has relied on
laborious and time-consuming breeding techniques. Genome-editing technologies have led …
laborious and time-consuming breeding techniques. Genome-editing technologies have led …
Boosting plant genome editing with a versatile CRISPR-Combo system
CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …
genome engineering in plants. However, these systems are mostly used separately, leaving …
[HTML][HTML] Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA
Editing plant genomes is technically challenging in hard-to-transform plants and usually
involves transgenic intermediates, which causes regulatory concerns. Here we report two …
involves transgenic intermediates, which causes regulatory concerns. Here we report two …
[HTML][HTML] A CRISPR/Cas9 toolkit for multiplex genome editing in plants
HL Xing, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …
Transgenerational CRISPR-Cas9 activity facilitates multiplex gene editing in allopolyploid wheat
The CRISPR-Cas9-based multiplexed gene editing (MGE) provides a powerful method to
modify multiple genomic regions simultaneously controlling different agronomic traits in …
modify multiple genomic regions simultaneously controlling different agronomic traits in …
[HTML][HTML] In planta Genome Editing in Commercial Wheat Varieties
Limitations for the application of genome editing technologies on elite wheat (Triticum
aestivum L.) varieties are mainly due to the dependency on in vitro culture and regeneration …
aestivum L.) varieties are mainly due to the dependency on in vitro culture and regeneration …
Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins
This protocol is an extension to: Nat. Protoc. 9, 2395–2410 (2014); doi: 10.1038/nprot.
2014.157; published online 18 September 2014 In recent years, CRISPR/Cas9 has …
2014.157; published online 18 September 2014 In recent years, CRISPR/Cas9 has …