Micrographs of mouse liver gap junctions, isolated with detergents, and negatively stained
with uranyl acetate, have been recorded by low-irradiation methods. Our Fourier-averaged …

Micrographs of isolated gap junction specimens, negatively stained with one molybdate,
three tungstate and three uranyl stains, were recorded at low and high irradiation. Fourier …

X-ray crystallographic methods and electron microscope image analysis have been used to
correlate the structure and the chemical composition of gap junction plaques isolated intact …

Models for the spatial distribution of protein, lipid and water in gap junction structures have
been constructed from the results of the analysis of X-ray diffraction data described here and …

We have studied the stain distribution within rat liver gap junctions for specimens prepared
by thin sectioning and negative staining. Pools of stain molecules exist in two specific …

Gap junctions, containing regular hexagonal arrays of connexons, have been isolated from
rat liver. The projected structures of these gap junctions have been studied to a resolution of …

Correlation of structural changes in isolated gap junctions with the mechanism of channel
gating is complicated by the effects of isolation procedures and the lack of a direct functional …

The structures of gap junctions isolated from mouse livers using two different protocols have
been studied using x-ray diffraction. The second protocol differs from the first in using the …

Publisher Summary In this chapter, an attempt is made to reconcile the contradictory images
of gap junctions observed by different experimental probes. The evidence for structure on …

This paper reports methods we have developed to solubilize gap junction channels, or
connexons, from isolated gap junctions and to purify them in milligram quantities. Two …