[HTML][HTML] An engineered hyaluronan synthase: characterization of recombinant human hyaluronan synthase 2 expressed in Escherichia coli
H Hoshi, H Nakagawa, S Nishiguchi, K Iwata… - Journal of Biological …, 2004 - ASBMB
The Class I hyaluronan synthase (HAS) is a unique glycosyltransferase synthesizing
hyaluronan (HA), a polysaccharide composed of GlcUA and GlcNAc, by using one catalytic …
hyaluronan (HA), a polysaccharide composed of GlcUA and GlcNAc, by using one catalytic …
[HTML][HTML] Three isoforms of mammalian hyaluronan synthases have distinct enzymatic properties
N Itano, T Sawai, M Yoshida, P Lenas… - Journal of Biological …, 1999 - ASBMB
Three mammalian hyaluronan synthase genes, HAS1, HAS2, and HAS3, have recently
been cloned. In this study, we characterized and compared the enzymatic properties of …
been cloned. In this study, we characterized and compared the enzymatic properties of …
[HTML][HTML] Hyaluronan synthases: a decade-plus of novel glycosyltransferases
PH Weigel, PL DeAngelis - Journal of Biological Chemistry, 2007 - ASBMB
Hyaluronan synthases (HASs) are glycosyltransferases that catalyze polymerization of
hyaluronan found in vertebrates and certain microbes. HASs transfer two distinct …
hyaluronan found in vertebrates and certain microbes. HASs transfer two distinct …
[HTML][HTML] Enzymological Characterization of Recombinant XenopusDG42, A Vertebrate Hyaluronan Synthase
PE Pummill, AM Achyuthan, PL DeAngelis - Journal of Biological Chemistry, 1998 - ASBMB
We have characterized the hyaluronan (HA) synthase activity of the Xenopus DG42 gene
product in vitro. The recombinant enzyme produced in yeast does not possess a nascent HA …
product in vitro. The recombinant enzyme produced in yeast does not possess a nascent HA …
[HTML][HTML] Biosynthesis of hyaluronan: direction of chain elongation
S Bodevin-Authelet, M Kusche-Gullberg… - Journal of Biological …, 2005 - ASBMB
Hyaluronan (HA), a functionally essential glycosaminoglycan in vertebrate tissues and a
putative virulence factor in certain pathogenic bacteria, is an extended linear polymer …
putative virulence factor in certain pathogenic bacteria, is an extended linear polymer …
Hyaluronan synthase polymerizing activity and control of product size are discrete enzyme functions that can be uncoupled by mutagenesis of conserved cysteines
PH Weigel, BA Baggenstoss - Glycobiology, 2012 - academic.oup.com
Streptococcus equisimilis hyaluronan (HA) synthase (SeHAS) contains four cysteines
(C226, C262, C281 and C367) that are conserved in the mammalian HAS family. Previous …
(C226, C262, C281 and C367) that are conserved in the mammalian HAS family. Previous …
In Vitro Assay for Hyaluronan Synthase
AP Spicer - Proteoglycan Protocols, 2001 - Springer
Hyaluronan (HA) is synthesized at the plasma membrane as a free linear polymer of
composition [β1→ 4GlcAβ1→ 3GlcNAc] n (1–3). All models suggest that polymerization …
composition [β1→ 4GlcAβ1→ 3GlcNAc] n (1–3). All models suggest that polymerization …
[HTML][HTML] Molecular cloning and characterization of a putative mouse hyaluronan synthase
AP Spicer, ML Augustine, JA McDonald - Journal of Biological Chemistry, 1996 - ASBMB
We report the isolation of a novel mouse gene which encodes a putative hyaluronan
synthase. The cDNA was identified using degenerate reverse transcriptase-polymerase …
synthase. The cDNA was identified using degenerate reverse transcriptase-polymerase …
[HTML][HTML] Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase
AP Spicer, JS Olson, JA McDonald - Journal of Biological Chemistry, 1997 - ASBMB
We report the isolation of a cDNA encoding the third putative hyaluronan synthase, HAS3.
Partial cDNAs and genomic fragments of mouse Has3 were obtained using a degenerate …
Partial cDNAs and genomic fragments of mouse Has3 were obtained using a degenerate …
An enzyme capture assay for analysis of active hyaluronan synthases
Z Kyossev, PH Weigel - Analytical biochemistry, 2007 - Elsevier
We describe a sensitive assay for detection of active hyaluronan synthases (HASs) capable
of synthesizing hyaluronan (HA) without use of radioactive uridine 5′-diphosphate sugar …
of synthesizing hyaluronan (HA) without use of radioactive uridine 5′-diphosphate sugar …