It is highly desirable to identify gene's function in a high-throughput fashion, and the
CRISPR/Cas9 system has been harnessed to meet such a need. Here, we describe a …

Identification of genes influencing a phenotype of interest is frequently achieved through
genetic screening by RNA interference (RNAi) or knockouts. However, RNAi may only …

CRISPR/Cas9 technology is ideally suited for genome‐wide screening applications due to
the ease of generating guide RNAs (gRNAs) and the versatility of Cas9 or Cas9 derivatives …

Forward genetic screens are powerful tools for the unbiased discovery and functional
characterization of specific genetic elements associated with a phenotype of interest …

To date, lentiviral-based CRISPR-Cas9 screens have largely been conducted in pooled
format. However, numerous assays are not amenable to pooled approaches, and lentiviral …

The ability to systematically disrupt genes serves as a powerful tool for understanding their
function. The programmable CRISPR–Cas9 system enables efficient targeting of large …

Background In recent years, large-scale genetic screens using the CRISPR/Cas9 system
have emerged as scalable approaches able to interrogate gene function with …

CRISPR-Cas9 technology provides a simple way to introduce targeted mutations into
mammalian cells to induce loss-of-function phenotypes. The CRISPR-Cas9 system has now …

Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR
technology in vivo. Here, we present a protocol for genome engineering using selective …

We developed an in vivo library-on-library methodology to simultaneously assess single
guide RNA (sgRNA) activity across∼ 1,400 genomic loci. Assaying across multiple human …