[HTML][HTML] Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing—Enabling robust and non-invasive biomarker research
Small extracellular vesicles (EVs) are 50–200 nm sized mediators in intercellular
communication that reflect both physiological and pathophysiological changes of their …
communication that reflect both physiological and pathophysiological changes of their …
Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development
Extracellular vesicles (EVs) have great potential as a source for clinically relevant
biomarkers since they can be readily isolated from biofluids and carry microRNA (miRNA) …
biomarkers since they can be readily isolated from biofluids and carry microRNA (miRNA) …
A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated extracellular vesicle enrichment by acoustic trapping to microRNA sequencing
Development of a robust automated platform for enrichment of extracellular vesicles from
low sample volume that matches the needs for next-generation sequencing could remove …
low sample volume that matches the needs for next-generation sequencing could remove …
Urinary extracellular vesicles for RNA extraction: optimization of a protocol devoid of prokaryote contamination
Background Urinary extracellular vesicles (UEVs) represent an ideal platform for biomarker
discovery. They carry different types of RNA species, and reported profile discrepancies …
discovery. They carry different types of RNA species, and reported profile discrepancies …
Urinary extracellular vesicles: Assessment of pre‐analytical variables and development of a quality control with focus on transcriptomic biomarker research
K Barreiro, OP Dwivedi, S Valkonen… - Journal of …, 2021 - Wiley Online Library
Urinary extracellular vesicles (uEV) are a topical source of non‐invasive biomarkers for
health and diseases of the urogenital system. However, several challenges have become …
health and diseases of the urogenital system. However, several challenges have become …
MicroRNA profiling of low concentration extracellular vesicle RNA utilizing NanoString nCounter technology
RE Crossland, A Albiero… - Journal of …, 2023 - Wiley Online Library
Extracellular vesicles (EV) and the microRNAs that they contain are increasingly recognised
as a rich source of informative biomarkers, reflecting pathological processes and …
as a rich source of informative biomarkers, reflecting pathological processes and …
Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery
Circulating miRNAs are detected in extracellular space and body fluids such as urine.
Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and …
Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and …
Comparative miRNA analysis of urine extracellular vesicles isolated through five different methods
F Royo, I Diwan, MR Tackett, P Zuñiga… - Cancers, 2016 - mdpi.com
Urine extracellular vesicles are a valuable low-invasive source of information, especially for
the cells of the genitourinary tract. In the search for biomarkers, different techniques have …
the cells of the genitourinary tract. In the search for biomarkers, different techniques have …
MicroRNAs in extracellular vesicles: sorting mechanisms, diagnostic value, isolation, and detection technology
D Xu, K Di, B Fan, J Wu, X Gu, Y Sun… - … in Bioengineering and …, 2022 - frontiersin.org
MicroRNAs (miRNAs) are a class of short, single-stranded, noncoding RNAs, with a length
of about 18–22 nucleotides. Extracellular vesicles (EVs) are derived from cells and play a …
of about 18–22 nucleotides. Extracellular vesicles (EVs) are derived from cells and play a …
Profiling of extracellular small RNAs highlights a strong bias towards non-vesicular secretion
The extracellular environment consists of a plethora of molecules, including extracellular
miRNA that can be secreted in association with extracellular vesicles (EVs) or soluble …
miRNA that can be secreted in association with extracellular vesicles (EVs) or soluble …