[HTML][HTML] Comparing small urinary extracellular vesicle purification methods with a view to RNA sequencing—Enabling robust and non-invasive biomarker research

V Mussack, G Wittmann, MW Pfaffl - Biomolecular Detection and …, 2019 - Elsevier
Small extracellular vesicles (EVs) are 50–200 nm sized mediators in intercellular
communication that reflect both physiological and pathophysiological changes of their …

Assessment of methods for serum extracellular vesicle small RNA sequencing to support biomarker development

S Srinivasan, MX Duval, V Kaimal, C Cuff… - Journal of extracellular …, 2019 - Taylor & Francis
Extracellular vesicles (EVs) have great potential as a source for clinically relevant
biomarkers since they can be readily isolated from biofluids and carry microRNA (miRNA) …

A urinary extracellular vesicle microRNA biomarker discovery pipeline; from automated extracellular vesicle enrichment by acoustic trapping to microRNA sequencing

A Ku, N Ravi, M Yang, M Evander, T Laurell, H Lilja… - PLoS …, 2019 - journals.plos.org
Development of a robust automated platform for enrichment of extracellular vesicles from
low sample volume that matches the needs for next-generation sequencing could remove …

Urinary extracellular vesicles for RNA extraction: optimization of a protocol devoid of prokaryote contamination

D Tataruch-Weinert, L Musante, O Kretz… - Journal of extracellular …, 2016 - Taylor & Francis
Background Urinary extracellular vesicles (UEVs) represent an ideal platform for biomarker
discovery. They carry different types of RNA species, and reported profile discrepancies …

Urinary extracellular vesicles: Assessment of pre‐analytical variables and development of a quality control with focus on transcriptomic biomarker research

K Barreiro, OP Dwivedi, S Valkonen… - Journal of …, 2021 - Wiley Online Library
Urinary extracellular vesicles (uEV) are a topical source of non‐invasive biomarkers for
health and diseases of the urogenital system. However, several challenges have become …

MicroRNA profiling of low concentration extracellular vesicle RNA utilizing NanoString nCounter technology

RE Crossland, A Albiero… - Journal of …, 2023 - Wiley Online Library
Extracellular vesicles (EV) and the microRNAs that they contain are increasingly recognised
as a rich source of informative biomarkers, reflecting pathological processes and …

Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery

AH Gheinani, M Vögeli, U Baumgartner, E Vassella… - Scientific reports, 2018 - nature.com
Circulating miRNAs are detected in extracellular space and body fluids such as urine.
Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and …

Comparative miRNA analysis of urine extracellular vesicles isolated through five different methods

F Royo, I Diwan, MR Tackett, P Zuñiga… - Cancers, 2016 - mdpi.com
Urine extracellular vesicles are a valuable low-invasive source of information, especially for
the cells of the genitourinary tract. In the search for biomarkers, different techniques have …

MicroRNAs in extracellular vesicles: sorting mechanisms, diagnostic value, isolation, and detection technology

D Xu, K Di, B Fan, J Wu, X Gu, Y Sun… - … in Bioengineering and …, 2022 - frontiersin.org
MicroRNAs (miRNAs) are a class of short, single-stranded, noncoding RNAs, with a length
of about 18–22 nucleotides. Extracellular vesicles (EVs) are derived from cells and play a …

Profiling of extracellular small RNAs highlights a strong bias towards non-vesicular secretion

H Sork, M Conceicao, G Corso, J Nordin, YXF Lee… - Cells, 2021 - mdpi.com
The extracellular environment consists of a plethora of molecules, including extracellular
miRNA that can be secreted in association with extracellular vesicles (EVs) or soluble …