In some lysosomal storage diseases (LSD) cholesterol accumulates in vesicles. Whether
increased vesicle cholesterol affects vesicle fusion with the plasmalemma, where the fusion …

The function of lysosomes relies on the ability of the lysosomal membrane to fuse with
several target membranes in the cell. It is known that in lysosomal storage disorders (LSDs) …

SNARE-mediated membrane fusion proceeds via the formation of a fusion pore. This
intermediate structure is highly dynamic and can flicker between open and closed states. In …

Membrane organization has received substantial research interest since the degree of
ordering in membrane regions is relevant in many biological processes. Here we relate the …

Since the 1970s, much effort was been expended researching mechanisms of regulated
exocytosis. Early work focused mainly on the role of proteins. Most notably the discovery of …

The process of regulated exocytosis is defined by the Ca2+-triggered fusion of two apposed
membranes, enabling the release of vesicular contents. This fusion step involves a number …

Cholesterol is an important lipid component of cellular membranes. Recent studies have
shown that changes in cellular cholesterol level can affect cellular functions. Here, we …

Cholesterol is essential for neuronal activity and function. Cholesterol depletion in the
plasma membrane impairs synaptic transmission. However, the molecular mechanisms by …

Freeze-fracture electron micrographs from degranulating cells show that the lumen of the
secretory granule is connected to the extracellular compartment via large (20 to 150 nm …

Membrane microdomains orrafts' are suggested to act as regulators of the exocytotic
process and also appear to be the sites of Ca2+-triggered membrane fusion. Microdomains …