Detection of human cytomegalovirus DNA: how, when and where?

G Gerna, F Baldanti, D Zella… - Scandinavian Journal of …, 1995 - europepmc.org
Although several methods have been utilized for the detection and quantification of human
cytomegalovirus (HCMV) DNA, all of them can be divided into three groups:(i) detection of …

Identification of human cytomegalovirus isolates by the polymerase chain reaction.

D Zipeto, E Silini, M Parea, E Percivalle… - Microbiologica, 1990 - europepmc.org
Fifty human cytomegalovirus (HCMV) isolates were recovered from different clinical
specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 AIDS …

Detection of human cytomegalovirus in urine by DNA-DNA and RNA-DNA hybridization

V Schuster, B Matz, H Wiegand, B Traub… - Journal of Infectious …, 1986 - academic.oup.com
A diagnostic hybridization assay for detecting human cytomegalovirus (HCMV) DNA in urine
specimens was developed by using cloned viral DNA and in vitro—synthesized RNA …

Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples …

D Zipeto, MG Revello, E Silini, M Parea… - Journal of Clinical …, 1992 - Am Soc Microbiol
The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the
polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised …

Polymerase chain reaction assay for detection of human cytomegalovirus

DM Olive, M Simsek, S Al-Mufti - Journal of Clinical Microbiology, 1989 - Am Soc Microbiol
Direct detection of human cytomegalovirus (HCMV) from clinical specimens was examined
by using the polymerase chain reaction (PCR) for amplifying HCMV DNA. The efficiency of …

Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction

K Hsia, DH Spector, J Lawrie… - Journal of Clinical …, 1989 - Am Soc Microbiol
Polymerase chain reaction (PCR) amplification was used to detect human cytomegalovirus
(HCMV) sequences. The fragments selected for amplification were fragments of 130 and …

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

RD Allen, PE Pellett, JA Stewart… - Journal of clinical …, 1995 - Am Soc Microbiol
We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus
(HCMV). The assay includes primer and probe sequences derived from conserved HCMV …

Polymerase chain reaction detection of human cytomegalovirus in over 2000 blood specimens correlated with virus isolation and related to urinary virus excretion

E Drouet, S Michelson, G Denoyel… - Journal of virological …, 1993 - Elsevier
The polymerase chain reaction (PCR) as applied to human cytomegalovirus (HCMV)
detection should provide a valuable tool for rapid, reliable diagnosis of infection, thereby …

Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections

M Brytting, W Xu, B Wahren… - Journal of clinical …, 1992 - Am Soc Microbiol
Using a specific and sensitive polymerase chain reaction method, we detected reliably the
presence of human cytomegalovirus (HCMV) DNA directly in serum samples collected at an …

Automated extraction and quantification of human cytomegalovirus DNA in whole blood by real-time PCR assay

C Mengelle, K Sandres-Saune… - Journal of clinical …, 2003 - Am Soc Microbiol
The measurement of human cytomegalovirus (HCMV) DNA in blood is becoming the
standard method for monitoring HCMV infection in immune-suppressed and unsuppressed …