Fluorescence method for measuring the kinetics of calcium ion-induced phase separations in phosphatidylserine-containing lipid vesicles

D Hoekstra - Biochemistry, 1982 - ACS Publications
Dick Hoekstra abstract: The effects of Ca2+ and Mg2+ on the fluorescence behavior of the
phospholipid analogues 1-acyl-2-[6-[(7-nitro-2, 1, 3-benzoxadiazol-4-yl) amino] caproyl] …

Studies on the mechanism of membrane fusion: role of phosphate in promoting calcium ion induced fusion of phospholipid vesicles

R Fraley, J Wilschut, N Duzgunes, C Smith… - Biochemistry, 1980 - ACS Publications
Robert Fraley,* Jan Wilschut, 8 Nejat Duzgunes, 1 Craig Smith, and Demetrios
Papahadjopoulos* abstract: The role of phosphate in enhancing the calciuminduced fusion …

Studies on the mechanism of membrane fusion. Role of head-group composition in calcium-and magnesium-induced fusion of mixed phospholipid vesicles

N Düzgünes, J Wilschut, R Fraley… - … et Biophysica Acta (BBA …, 1981 - Elsevier
We have investigated the contribution of various phospholipids to membrane fusion induced
by divalent cations. Fusion was followed by means of a new fluorescence assay monitoring …

Role of lipid phase separations and membrane hydration in phospholipid vesicle fusion

D Hoekstra - Biochemistry, 1982 - ACS Publications
Dick Hoekstra** abstract: The relationship between lipid phase separation and fusion of
small unilamellar phosphatidylserine-containing vesicles was investigated. The kinetics of …

Monitoring of phospholipid vesicle fusion by fluorescence energy transfer between membrane-bound dye labels

P Vanderwerf, EF Ullman - Biochimica et Biophysica Acta (BBA) …, 1980 - Elsevier
A sensitive method which utilizes fluorescence energy transfer to assay Ca 2+-or Mg 2+-
mediated fusion of phospholipid vesicles is reported. More than 85% quenching results …

Ca2+-induced fusion of phosphatidylserine vesicles: mass action kinetic analysis of membrane lipid mixing and aqueous contents mixing

J Wilschut, J Scholma, M Bental, D Hoekstra… - Biochimica et Biophysica …, 1985 - Elsevier
We have investigated the initial kinetics of Ca 2+-induced aggregation and fusion of
phosphatidylserine large unilamellar vesicles at 3, 5 and 10 mM Ca 2+ and 15, 25 and 35° …

Retention of aqueous contents during divalent cation-induced fusion of phospholipid vesicles

J Wilschut, N Düzgünes, K Hong, D Hoekstra… - … et Biophysica Acta (BBA …, 1983 - Elsevier
The relative kinetics of intermixing and release of liposome aqueous contents during Ca 2+-
induced membrane fusion has been investigated. Fusion was monitored by the Tb …

Lipid phase behavior and calcium-induced fusion of phosphatidylethanolamine-phosphatidylserine vesicles. Calorimetric and fusion studies

JR Silvius, J Gagne - Biochemistry, 1984 - ACS Publications
John R. Silvius* and Jeannine Gagné abstract: Pure dioleoyl-, dielaidoyl-, and dimyristoyl-
phosphatidylserines havebeen synthesized, along with the corresponding …

Membrane fusion and molecular segregation in phospholipid vesicles

D Papahadjopoulos, G Poste, BE Schaeffer… - Biochimica et biophysica …, 1974 - Elsevier
Fusion between vesicles prepared from individual or mixed phospholipid species was
demonstrated by ultracentrifugation and gel-filtration techniques, electron microscopy and …

Modulation of membrane fusion by membrane fluidity: temperature dependence of divalent cation induced fusion of phosphatidylserine vesicles

J Wilschut, N Duzgunes, D Hoekstra… - Biochemistry, 1985 - ACS Publications
Revised Manuscript Received August 24, 1984 abstract: We have investigated
thetemperature dependence of the fusion of phospholipid vesicles composed of pure bovine …