It has been known that the mammalian ovary contains a huge number of non-growing small
oocytes, of which only a small number grow to their final size, mature, and are ovulated …

This article summarizes recent progress in reproductive biotechnology in swine with special
reference to in vitro production of embryos, generation of identical multiples, and transgenic …

In the present study, attempts were made to develop a protein-free (PF) in vitro maturation
(IVM) system for pig oocytes and to examine subsequent embryo development after in vitro …

To clone a pig from somatic cells, we first validated an electrical activation method for use on
ovulated oocytes. We then evaluated delayed versus simultaneous activation (DA vs. SA) …

To induce meiotic resumption of porcine oocytes, it is thought to be necessary to expose the
cumulus-oocyte complexes (COCs) to gonadotropins during in-vitro maturation (IVM) …

A series of integrated, effective techniques is required to produce pig embryos from follicular
oocytes in vitro. The failure to form a male pronucleus and polyspermy have been serious …

To establish a reliable in vitro maturation system for activation and subsequent development
as nuclear recipients for the effective production of pig clones, we assessed maturation …

We report the successful piglet production from cryopreserved oocytes for the first time by
using a simple, high capacity vitrification protocol for preservation and a defined system for …

In livestock, most of the follicles on the ovarian surface are small follicles. A procedure that
supports the in vitro growth and maturation of these small follicle-derived oocytes may offer a …

The objective of this study was to assess the practical usefulness of morphologically poor
oocytes (MPCOCs) in relation to follicular size and oocyte diameter. Oocytes collected from …