This study examined the ability of epidermal growth factor (EGF) to improve the
developmental competence of pig oocytes matured in a protein-free (PF) in vitro maturation …

In recent years, progress has been achieved in the production of pig embryos through IVM
and IVF techniques. Cytoplasmic maturation of oocytes has been improved by modifications …

Follicular oocytes collected from prepubertal gilts at a local slaughter house were matured
(36 h), fertilized and developed in vitro. Of 785 embryos, 190 (24%) embryos cleaved to the …

Contents This paper summarizes the major advances in farm animal assisted reproduction
in the last 20 years with particular attention to the contributions of the authors. A main …

The establishment of in vitro fertilization and culture systems for mammalian embryos has
facilitated the application of embryo technologies in research, industry, and clinical settings …

Assisted reproduction technology (ART) protocols are used in livestock for the improvement
and preservation of their genetics and to enhance reproductive efficiency. In the case of …

The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low.
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …

The type of donor cell most suitable for producing cloned animals is one of the topics under
debate in the field of nuclear transfer. To provide useful information to answer this question …

Although pronuclear DNA micro-injection has long been the most reliable method to
produce transgenic pigs, the efficiency of production of transgenic offspring is generally …

Growth factors were studied as a means of increasing the development of in vitro matured
(IVM) and in vitro fertilized (IVF) oocytes into morulae or blastocysts. Cell numbers of …