The ability to visualize neurons inside living brain tissue is a fundamental requirement in
neuroscience and neurosurgery. Especially the development of a noninvasive probe of …

Multiphoton microscopy relies on nonlinear light–matter interactions to provide contrast and
optical sectioning capability for high-resolution imaging. Most multiphoton microscopy …

Label-free microscopy is a very powerful technique that can be applied to study samples
with no need for exogenous fluorescent probes, keeping the main benefits of multiphoton …

Second harmonic generation (SHG) is inherently sensitive to the absence of spatial
centrosymmetry, which can render it intrinsically sensitive to interfacial processes, chemical …

In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative
assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an …

We find that several key endogenous protein structures give rise to intense second-
harmonic generation (SHG)—nonabsorptive frequency doubling of an excitation laser line …

A laser scanning microscope using third-harmonic generation as a probe is shown to
produce high-resolution images of transparent biological specimens. Third harmonic light is …

Second and Third Harmonic Generation (SHG and THG) microscopy is based on optical
effects which are induced by specific inherent physical properties of a specimen. As a multi …

Nonlinear optical imaging has revolutionized microscopy for the life sciences. Second
harmonic generation (SHG), the younger sibling of two-photon excited fluorescence (2PF), is …

In recent years, nonlinear laser scanning microscopy has gained much attention due to its
unique ability of deep optical sectioning. Based on our previous studies, a 1,200–1,300‐nm …