TrwC-mediated site-specific recombination is controlled by host factors altering local DNA topology
CE César, M Llosa - Journal of bacteriology, 2007 - Am Soc Microbiol
ABSTRACT R388 conjugative relaxase TrwC acts as a site-specific recombinase, promoting
recombination between two cognate oriT s on double-stranded DNA substrates. The …
recombination between two cognate oriT s on double-stranded DNA substrates. The …
A new domain of conjugative relaxase TrwC responsible for efficient oriT‐specific recombination on minimal target sequences
CE César, C Machón, F De La Cruz… - Molecular …, 2006 - Wiley Online Library
We show that relaxase TrwC promotes recombination between two directly repeated oriTs
while related relaxases TraI of F and pKM101 do not. Efficient recombination required also …
while related relaxases TraI of F and pKM101 do not. Efficient recombination required also …
Site-specific recombinase and integrase activities of a conjugative relaxase in recipient cells
O Draper, CE César, C Machon… - Proceedings of the …, 2005 - National Acad Sciences
Conjugative relaxases are the proteins that initiate bacterial conjugation by a site-specific
cleavage of the transferred DNA strand. In vitro, they show strand-transferase activity on …
cleavage of the transferred DNA strand. In vitro, they show strand-transferase activity on …
[HTML][HTML] Site-specific integration of foreign DNA into minimal bacterial and human target sequences mediated by a conjugative relaxase
L Agúndez, C González-Prieto, C Machón, M Llosa - PloS one, 2012 - journals.plos.org
Background Bacterial conjugation is a mechanism for horizontal DNA transfer between
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …
Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC
A Guasch, M Lucas, G Moncalián, M Cabezas… - Nature Structural & …, 2003 - nature.com
Relaxases are DNA strand transferases that catalyze the initial and final stages of DNA
processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer …
processing during conjugative cell-to-cell DNA transfer. Upon binding to the origin of transfer …
The conjugative relaxase TrwC promotes integration of foreign DNA in the human genome
C González-Prieto, R Gabriel, C Dehio… - Applied and …, 2017 - Am Soc Microbiol
Bacterial conjugation is a mechanism of horizontal DNA transfer. The relaxase TrwC of the
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …
IHF protein inhibits cleavage but not assembly of plasmid R388 relaxosomes
G Moncalián, M Valle, JM Valpuesta… - Molecular …, 1999 - Wiley Online Library
Relaxosomes are specific nucleoprotein structures involved in DNA‐processing reactions
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …
The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase
FX Gomis-RuÈth, G Moncalián, R Pérez-Luque… - Nature, 2001 - nature.com
The transfer of DNA across membranes and between cells is a central biological process;
however, its molecular mechanism remains unknown. In prokaryotes, trans-membrane …
however, its molecular mechanism remains unknown. In prokaryotes, trans-membrane …
Changing the recognition site of a conjugative relaxase by rational design
B González‐Pérez, JD Carballeira… - Biotechnology …, 2009 - Wiley Online Library
TrwC is a relaxase protein, which starts and finishes DNA processing during bacterial
conjugation in plasmid R388. TrwC recognizes a specific sequence of DNA (25 nucleotides) …
conjugation in plasmid R388. TrwC recognizes a specific sequence of DNA (25 nucleotides) …
Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides
Protein TrwC is the conjugative relaxase responsible for DNA processing in plasmid R388
bacterial conjugation. TrwC has two catalytic tyrosines, Y18 and Y26, both able to carry out …
bacterial conjugation. TrwC has two catalytic tyrosines, Y18 and Y26, both able to carry out …