A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation
JD Carballeira, B González-Pérez… - Nucleic acids …, 2014 - academic.oup.com
Relaxases act as DNA selection sieves in conjugative plasmid transfer. Most plasmid
relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is …
relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is …
Structure of TrwB, a gatekeeper in bacterial conjugation
FX Gomis-Rüth, M Coll - The International Journal of Biochemistry & Cell …, 2001 - Elsevier
Bacterial conjugation implies a trans-membrane passage of DNA, mediated by proteins
encoded in conjugative plasmids. This results in a spread of genetic information, including …
encoded in conjugative plasmids. This results in a spread of genetic information, including …
Stringent and Relaxed Recognition of oriT by Related Systems for Plasmid Mobilization: Implications for Horizontal Gene Transfer
S Jandle, R Meyer - Journal of bacteriology, 2006 - Am Soc Microbiol
The plasmids R1162 and pSC101 have origins of conjugative transfer (oriT s) and
corresponding relaxases that are closely related. The oriT s are made up of a highly …
corresponding relaxases that are closely related. The oriT s are made up of a highly …
Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the …
A Beranek, M Zettl, K Lorenzoni, A Schauer… - Journal of …, 2004 - Am Soc Microbiol
Coupling proteins (CPs) are present in type IV secretion systems of plant, animal, and
human pathogens and are essential for DNA transfer in bacterial conjugation systems. CPs …
human pathogens and are essential for DNA transfer in bacterial conjugation systems. CPs …
Location of the relaxation complex nick site within the minimal origin of transfer region of RK2
DG Guiney, C Deiss, V Simnad - Plasmid, 1988 - Elsevier
Transfer of plasmid DNA during bacterial conjugation begins at a specific site: the origin of
transfer (oriT). The oriT region of the broad host range plasmid RK2 is located on a 250 bp …
transfer (oriT). The oriT region of the broad host range plasmid RK2 is located on a 250 bp …
Site-directed mutations in the relaxase operon of RP4
SP Cole, E Lanka, DG Guiney - Journal of bacteriology, 1993 - Am Soc Microbiol
Mutations were constructed by site-directed mutagenesis in the relaxase operon of the
broad-host-range plasmid RP4. The mutations were constructed in smaller plasmids …
broad-host-range plasmid RP4. The mutations were constructed in smaller plasmids …
[HTML][HTML] Concerted action of three distinct domains in the DNA cleaving-joining reaction catalyzed by relaxase (TraI) of conjugative plasmid RP4.
W Pansegrau, W Schröder, E Lanka - Journal of Biological Chemistry, 1994 - Elsevier
The TraI protein of plasmid RP4 (IncP alpha) catalyzes a site-and strand-specific cleaving-
joining reaction on form I or single-stranded DNA. Thus, TraI is one of the key components …
joining reaction on form I or single-stranded DNA. Thus, TraI is one of the key components …
Identification and characterization of Thermus thermophilus HB8 RuvA protein, the subunit of the RuvAB protein complex that promotes branch migration of Holliday …
T Ohnishi, H Iwasaki, Y Ishino, S Kuramitsu… - Genes & genetic …, 2000 - jstage.jst.go.jp
The Escherichia coli ruvA and ruvB genes constitute an SOS-regulated operon. The
products of these genes form a protein complex that promotes branch migration of the …
products of these genes form a protein complex that promotes branch migration of the …
The secret life of conjugative relaxases
DL Guzmán-Herrador, M Llosa - Plasmid, 2019 - Elsevier
Conjugative relaxases are well-characterized proteins responsible for the site-and strand-
specific endonucleolytic cleavage and strand transfer reactions taking place at the start and …
specific endonucleolytic cleavage and strand transfer reactions taking place at the start and …
[HTML][HTML] Covalent association of the traI gene product of plasmid RP4 with the 5'-terminal nucleotide at the relaxation nick site.
W Pansegrau, G Ziegelin, E Lanka - Journal of Biological Chemistry, 1990 - Elsevier
Formation of relaxosomes is the first step in the initiation of transfer DNA replication during
bacterial conjugation. This nucleoprotein complex contains all components capable of …
bacterial conjugation. This nucleoprotein complex contains all components capable of …