Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes
M Llosa, S Zunzunegui… - Proceedings of the …, 2003 - National Acad Sciences
Conjugative coupling proteins (CPs) are proposed to play a role in connecting the
relaxosome to a type IV secretion system (T4SS) during bacterial conjugation. Here we …
relaxosome to a type IV secretion system (T4SS) during bacterial conjugation. Here we …
Molecular recognition determinants for type IV secretion of diverse families of conjugative relaxases
In preparation for transfer conjugative type IV secretion systems (T4SS) produce a
nucleoprotein adduct containing a relaxase enzyme covalently linked to the 5′ end of …
nucleoprotein adduct containing a relaxase enzyme covalently linked to the 5′ end of …
XerD-mediated FtsK-independent integration of TLCϕ into the Vibrio cholerae genome
As in most bacteria, topological problems arising from the circularity of the two Vibrio
cholerae chromosomes, chrI and chrII, are resolved by the addition of a crossover at a …
cholerae chromosomes, chrI and chrII, are resolved by the addition of a crossover at a …
Functional complementation of UvsX and UvsY mutations in the mediation of T4 homologous recombination
JN Farb, SW Morrical - Nucleic acids research, 2009 - academic.oup.com
Bacteriophage T4 homologous recombination events are promoted by presynaptic filaments
of UvsX recombinase bound to single-stranded DNA (ssDNA). UvsY, the phage …
of UvsX recombinase bound to single-stranded DNA (ssDNA). UvsY, the phage …
Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation
E Cabezon, JI Sastre, F De La Cruz - Molecular and General Genetics …, 1997 - Springer
The ability of conjugative plasmids from six different incompatibility groups to mobilize a set
of mobilizable plasmids was examined. The mobilization frequencies of plasmids RSF1010 …
of mobilizable plasmids was examined. The mobilization frequencies of plasmids RSF1010 …
A Novel Fold in the TraI Relaxase–Helicase C-Terminal Domain Is Essential for Conjugative DNA Transfer
LM Guogas, SA Kennedy, JH Lee… - Journal of molecular …, 2009 - Elsevier
TraI relaxase–helicase is the central catalytic component of the multiprotein relaxosome
complex responsible for conjugative DNA transfer (CDT) between bacterial cells. CDT is a …
complex responsible for conjugative DNA transfer (CDT) between bacterial cells. CDT is a …
Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins
A Varsaki, G Moncalián… - Journal of …, 2009 - Am Soc Microbiol
MbeC is a 13-kDa ColE1-encoded protein required for efficient mobilization of ColE1, a
plasmid widely used in cloning vector technology. MbeC protein was purified and used for in …
plasmid widely used in cloning vector technology. MbeC protein was purified and used for in …
Homology-dependent interactions determine the order of strand exchange by IntDOT recombinase
J Laprise, S Yoneji, JF Gardner - Nucleic acids research, 2010 - academic.oup.com
ABSTRACT The Bacteroides conjugative transposon CTnDOT encodes an integrase,
IntDOT, which is a member of the tyrosine recombinase family. Other members of this group …
IntDOT, which is a member of the tyrosine recombinase family. Other members of this group …
Characterization of IntA, a bidirectional site-specific recombinase required for conjugative transfer of the symbiotic plasmid of Rhizobium etli CFN42
R Hernández-Tamayo, C Sohlenkamp… - Journal of …, 2013 - Am Soc Microbiol
Site-specific recombination occurs at short specific sequences, mediated by the cognate
recombinases. IntA is a recombinase from Rhizobium etli CFN42 and belongs to the tyrosine …
recombinases. IntA is a recombinase from Rhizobium etli CFN42 and belongs to the tyrosine …
Localized denaturation of oriT DNA within relaxosomes of the broad‐host‐range plasmid R1162
S Zhang, RJ Meyer - Molecular microbiology, 1995 - Wiley Online Library
The broad‐host‐range, multicopy plasmid R1162 is efficiently mobilized during conjugation
by the self‐transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and …
by the self‐transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and …