Conjugative coupling proteins interact with cognate and heterologous VirB10-like proteins while exhibiting specificity for cognate relaxosomes

M Llosa, S Zunzunegui… - Proceedings of the …, 2003 - National Acad Sciences
Conjugative coupling proteins (CPs) are proposed to play a role in connecting the
relaxosome to a type IV secretion system (T4SS) during bacterial conjugation. Here we …

Molecular recognition determinants for type IV secretion of diverse families of conjugative relaxases

S Lang, K Gruber, S Mihajlovic, R Arnold… - Molecular …, 2010 - Wiley Online Library
In preparation for transfer conjugative type IV secretion systems (T4SS) produce a
nucleoprotein adduct containing a relaxase enzyme covalently linked to the 5′ end of …

XerD-mediated FtsK-independent integration of TLCϕ into the Vibrio cholerae genome

C Midonet, B Das, E Paly… - Proceedings of the …, 2014 - National Acad Sciences
As in most bacteria, topological problems arising from the circularity of the two Vibrio
cholerae chromosomes, chrI and chrII, are resolved by the addition of a crossover at a …

Functional complementation of UvsX and UvsY mutations in the mediation of T4 homologous recombination

JN Farb, SW Morrical - Nucleic acids research, 2009 - academic.oup.com
Bacteriophage T4 homologous recombination events are promoted by presynaptic filaments
of UvsX recombinase bound to single-stranded DNA (ssDNA). UvsY, the phage …

Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation

E Cabezon, JI Sastre, F De La Cruz - Molecular and General Genetics …, 1997 - Springer
The ability of conjugative plasmids from six different incompatibility groups to mobilize a set
of mobilizable plasmids was examined. The mobilization frequencies of plasmids RSF1010 …

A Novel Fold in the TraI Relaxase–Helicase C-Terminal Domain Is Essential for Conjugative DNA Transfer

LM Guogas, SA Kennedy, JH Lee… - Journal of molecular …, 2009 - Elsevier
TraI relaxase–helicase is the central catalytic component of the multiprotein relaxosome
complex responsible for conjugative DNA transfer (CDT) between bacterial cells. CDT is a …

Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins

A Varsaki, G Moncalián… - Journal of …, 2009 - Am Soc Microbiol
MbeC is a 13-kDa ColE1-encoded protein required for efficient mobilization of ColE1, a
plasmid widely used in cloning vector technology. MbeC protein was purified and used for in …

Homology-dependent interactions determine the order of strand exchange by IntDOT recombinase

J Laprise, S Yoneji, JF Gardner - Nucleic acids research, 2010 - academic.oup.com
ABSTRACT The Bacteroides conjugative transposon CTnDOT encodes an integrase,
IntDOT, which is a member of the tyrosine recombinase family. Other members of this group …

Characterization of IntA, a bidirectional site-specific recombinase required for conjugative transfer of the symbiotic plasmid of Rhizobium etli CFN42

R Hernández-Tamayo, C Sohlenkamp… - Journal of …, 2013 - Am Soc Microbiol
Site-specific recombination occurs at short specific sequences, mediated by the cognate
recombinases. IntA is a recombinase from Rhizobium etli CFN42 and belongs to the tyrosine …

Localized denaturation of oriT DNA within relaxosomes of the broad‐host‐range plasmid R1162

S Zhang, RJ Meyer - Molecular microbiology, 1995 - Wiley Online Library
The broad‐host‐range, multicopy plasmid R1162 is efficiently mobilized during conjugation
by the self‐transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and …