Using a combination of polyacrylamide gel electrophoresis in the presence of sodium
dodecyl sulfate and immunoblotting with antihuman GH (anti-hGH) serum, we quantitatively …

Several GH isoforms have been identified in pituitary and serum, the most abundant of
which is the 22K human GH (hGH) isoform. The 20K hGH isoform is produced by alternative …

Circulating human growth hormone (GH) consists of several molecular isoforms. Increased
proportion of circulating non-22K hGH and 20K hGH was reported in active acromegaly. In …

The hGH 20K variant accounts for 10-15% of the hGH in the pituitary gland and is, therefore,
the second most abundant hormone in the gland. 2. Although no specific RIA exists for hGH …

After pharmacological stimulation of the pituitary gland, human GH (hGH) in plasma consists
of three or more monomeric molecular forms and several corresponding oligomers …

A variant of human growth hormone (hGH), in which 15 amino acids are missing (commonly
referred to as 20K-hGH in contrast to the traditional form which is referred to as 22K-hGH), is …

Abstract The 20,000-dalton variant of human GH (hGH)(20 K) exhibits full growth-promoting
bioactivity in the rat despite its poor interaction with GH receptors, as compared to the …

We have derived and characterized a set of monoclonal antibodies (mAb) specific for the
different human GH (hGH) isoforms. The binding characteristics of each antibody to the hGH …

An isolation procedure for the 20,000-dalton variant of human growth hormone has been
devised to improve the yield of the final product. The improvement involved elimination of …

Human GH (hGH) extracted from pituitary glands consists of several molecular forms.
Monomeric pituitary forms include the single chain 22,000-dalton polypeptide (22K; hGH-B) …