Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Autotransporter proteins constitute a family of outer membrane/secreted proteins that
possess unique structural properties that facilitate their independent transport across the …

Autotransporters are widely distributed among Gram-negative bacteria. They can have a
large variety of functions and many of them have a role in virulence. They are synthesized as …

The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria
to export virulence and colonization factors to their cell surface or into their surroundings …

Background Cell display technologies (eg bacterial display) are attractive in directed
evolution as they provide the option to use flow-cytometric cell sorting for selection from …

Autotransporters are virulence‐related proteins of Gram‐negative bacteria that are secreted
via an outer‐membrane‐based C‐terminal extension, the translocator domain. This domain …

Autotransporters of gram-negative bacteria are single-peptide secretion systems that consist
of a functional N-terminal α-domain (“passenger”) fused to a C-terminal β-domain …