Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose

SA Schini, BD Bavister - Biology of reproduction, 1988 - academic.oup.com
The failure of hamster 2-cell embryos to develop in vitro (2-cell block) was examined with
experiments in which concentrations of glucose and phosphate in the culture medium were …

Phosphate is required for inhibition by glucose of development of hamster 8-cell embryos in vitro

PB Seshagiri, BD Bavister - Biology of reproduction, 1989 - academic.oup.com
The influence of sodium dihydrogen phosphate (Pi) and glucose on the development of
hamster 8-cell embryos mediated by pyruvate (P) or amino acids (A) or lactate (L) was …

Development of rat one-cell embryos in a chemically defined medium: effects of glucose, phosphate and osmolarity

K Miyoshi, H Funahashi, K Okuda, K Niwa - Reproduction, 1994 - rep.bioscientifica.com
Rat one-cell embryos recovered from naturally mated females were cultured in modified
hamster embryo culture medium 1 without amino acids. In the presence of 0.4 mmol …

Glucose inhibits development of hamster 8-cell embryos in vitro

PB Seshagiri, BD Bavister - Biology of reproduction, 1989 - academic.oup.com
Relative preferences of energy substrates (glucose, pyruvate, and lactate) for in vitro
development of hamster 8-cell embryos were investigated. Using protein-free modified …

An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro

CL Chatot, CA Ziomek, BD Bavister, JL Lewis… - …, 1989 - rep.bioscientifica.com
One-cell CF-1× B 6 SJLF 1/J embryos, which usually exhibit a 2-cell block to development in
vitro, have been cultured to the blastocyst stage using CZB medium and a glucose washing …

Inhibitory effect of glucose and phosphate on the second cleavage division of hamster embryos: is it linked to metabolism?

DK Barnett, BD Bavister - Human reproduction, 1996 - academic.oup.com
Hamster embryos cannot complete the second cleavage division in vitro in the presence of
glucose and inorganic phosphate (Glu/Pi). Embryos can recover from an 8 h (12: 30–20: 30 …

Energy substrate requirements for in-vitro development of hamster 1-and 2-cell embryos to the blastocyst stage

SH McKiernan, BD Bavister, RJ Tasca - Human reproduction, 1991 - academic.oup.com
Energy substrate requirements (pyruvate, lactate and amino acids) were determined for in-
vitro development of hamster 1-and 2-cell embryos to blastocysts, using a chemically …

Glucose and phosphate inhibit respiration and oxidative metabolism in cultured hamster eight‐cell embryos: Evidence for the “Crabtree effect”

PB Seshagiri, BD Bavister - Molecular reproduction and …, 1991 - Wiley Online Library
The development of hamster eight‐cell embryos is inhibited by glucose in culture medium
containing inorganic phosphate (Pi). This is hypothetically attributed to the “Crabtree effect,” …

Influence of single amino acids on the development of hamster one‐cell embryos in vitro

BD Bavister, T Arlotto - Molecular Reproduction and …, 1990 - Wiley Online Library
One‐cell hamster embryos placed in culture have always shown a complete block to
development at the two‐cell stage. In a preliminary study using a chemically defined culture …

Development of eight-cell mouse embryos in vitro

RL Brinster, JL Thomson - Experimental cell research, 1966 - Elsevier
For the two-cell mouse embryo to develop into a blastocyst in vitro, the culture medium must
contain both an energy source and a fixed nitrogen source. However, under the same …