The regulation of 1-cell mouse embryo development in culture by amino acids was
investigated. When the 20 amino acids in Eagle's medium were present, blastocyst …

The cleavage, metabolism and viability of mouse zygotes were assessed after culture in
media of different ionic and metabolite composition. Medium with a high potassium …

Embryos from certain strains of mice do not develop into blastocysts when cultured in vitro
from the 1-or 2-cell stages but arrest development as 2-cell embryos-a phenomenon …

Rat pronuclear embryos were cultured in hamster embryo culture medium-1 (HECM-1) or a
modified Krebs-Ringer bicarbonate solution (mKRB). Embryo cultures in HECM-1 were also …

We have shown in previous studies that development of 8-cell hamster embryos to hatching
and hatched blastocysts in vitro is stimulated by the addition to the culture medium of a …

Rat embryos at the head-fold stage were cultured in rotating bottles for 2 days in rat serum
extensively dialysed against glucose-free BSS, and to which various energy sources and …

The activities of four enzymes were determined during the first four days of mouse
embryogenesis. Two enzymes, fructose 1, 6-diphosphate aldolase and malate …

Simple media were developed to study the metabolic requirements of bovine embryos up to
Day 7 (Day 0= day of oocyte aspiration) in vitro. Embryos were derived from oocytes …

Although human embryos will develop in vitro for six days or more, little is known about the
effects of the primary nutrients, pyruvate and glucose, on development. Because the nutrient …

Three experiments were conducted in which 2-cell bovine embryos were prepared from
oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by …