Tn10 and IS10 transposition and chromosome rearrangements: mechanism and regulation in vivo and in vitro
N Kleckner, RM Chalmers, D Kwon, J Sakai… - Transposable …, 1996 - Springer
Tn10 is a composite transposon. It comprises a pair of IS10 insertion sequences located in
opposite orientation flanking~ 6.7 kb of unique sequences; these unique sequences encode …
opposite orientation flanking~ 6.7 kb of unique sequences; these unique sequences encode …
All three residues of the Tn10 transposase DDE catalytic triad function in divalent metal ion binding
JS Allingham, PA Pribil, DB Haniford - Journal of molecular biology, 1999 - Elsevier
Tn10/IS10 transposition involves excision of the transposon from a donor site and
subsequent joining of the excised transposon to a new target site. These steps are catalyzed …
subsequent joining of the excised transposon to a new target site. These steps are catalyzed …
The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD
The excision of transposon Tn7 from a donor site and its insertion into its preferred target
site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA …
site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA …
Analysis of Tn7 transposition
M Rogers, N Ekaterinaki, E Nimmo… - Molecular and General …, 1986 - Springer
Five gene products required for Tn 7 transposition were identified using genetic
complementation tests. Four of these (tns A, tnsB, tnsC and tnsD) are essential for insertion …
complementation tests. Four of these (tns A, tnsB, tnsC and tnsD) are essential for insertion …
Substrate recognition and induced DNA deformation by transposase at the target-capture stage of Tn10 transposition
PA Pribil, DB Haniford - Journal of Molecular Biology, 2000 - Elsevier
The bacterial transposon Tn10 inserts preferentially into sites that conform to a 9 bp
consensus sequence: 5′ NGCTNAGCN 3′. However, this sequence is not on its own …
consensus sequence: 5′ NGCTNAGCN 3′. However, this sequence is not on its own …
Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.
C Egner, DE Berg - … of the National Academy of Sciences, 1981 - National Acad Sciences
The excision of Tn5 from sites of insertion in the Escherichia coli genome was studied by
examining the reversion of lac:: Tn5 insertion mutations to lac+. We find that:(i) the frequency …
examining the reversion of lac:: Tn5 insertion mutations to lac+. We find that:(i) the frequency …
The TN5 transposon
WS Reznikoff - Annual review of microbiology, 1993 - go.gale.com
The element IS50 encodes all Tn5 functions required for transposition and all regulatory
mechanisms. Bacterial transposon Tn5 encodes two proteins, transposase and the …
mechanisms. Bacterial transposon Tn5 encodes two proteins, transposase and the …
Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 …
M Zhou, A Bhasin, WS Reznikoff - Journal of molecular biology, 1998 - Elsevier
Transposition of Tn5 and IS50 requires the specific binding of transposase (Tnp) to the end
inverted repeats, the outside end (OE) and the inside end (IE). OE and IE have 12 identical …
inverted repeats, the outside end (OE) and the inside end (IE). OE and IE have 12 identical …
Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase
P Avila, F De La Cruz, E Ward, J Grinsted - Molecular and General …, 1984 - Springer
In the presence of the Tn 21 transposase, plasmids that contain a single Tn 21 inverted
repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is …
repeat sequence fuse efficiently with other plasmids. This reaction occurs in recA strains, is …