Background: The use of three-dimensional in vitro models of brain tumor invasion has
provided a system for reconstructing some of the cellular microenvironments present in the …

✓ Tumor tissue from seven human gliomas was maintained in long-term agar overlay culture
as multicellular organotypic spheroids. Light microscopic and ultrastructural observation of …

Purpose. The primary cause of local recurrence and therapeutic failure in the treatment of
malignant gliomas is the invasion of tumor cells into the surrounding normal brain. While it is …

Owing to their in vivo-like characteristics, three-dimensional (3 D) multicellular tumor
spheroid (MCTS) cultures are gaining increasing popularity as an in vitro model of tumors. A …

Object. The aim of this study was to develop an improved animal model for brain tumor
study. The need for better and more relevant brain tumor models is generally acknowledged …

✓ A three-dimensional model has been developed in which C6 astrocytoma spheroids of
defined sizes are embedded into collagen type I gels. The authors have monitored cell …

Certain limitations of the neurosphere assay (NSA) have resulted in a search for alternative
culture techniques for brain tumor-initiating cells (TICs). Recently, reports have described …

A novel model of glioma cell invasion was established by using an organotypic culture of rat
brains. Brain slices prepared from 2-day-old rat neonates were maintained in a culture at the …

During the last three decades multicellular spheroids of both normal and malignant cells
have been used as model systems for studying three-dimen sional growth and differentiation …

The C6 spheroid implantation glioma model is a simple, easily reproduced model for
primary gliomas in which C6 astrocytoma cells are grown in vitro as spheroids and …