Liquid marbles are exploited in this study as efficient miniature bioreactors inoculated with
Hep G2 (hepatocellular carcinoma) cells to culture cell spheroids in vitro. This method …

Background The poor prognosis for glioblastoma patients is caused by the diffuse infiltrative
growth pattern of the tumor. Therefore, the molecular and cellular processes underlying cell …

To obtain a multicellular MCF-7 spheroid model to mimic the three-dimensional (3D) of
tumors, the microwell liquid overlay (A) and hanging-drop/agar (B) methods were first …

In vitro characterization of tumor cell biology or of potential anticancer drugs is usually
performed using tumor cell lines cultured as a monolayer. However, it has been previously …

The purpose of the meeting was to exchange information on spheroid culture methods and
to review the role of spheroids in cancer research. Techniques and results presented at this …

It is becoming more and more apparent that monolayer cultures of tumor cells cannot
completely represent the characteristics of three-dimensional solid tumors. Consequently …

The multicellular tumor spheroid model is a widely used culture system to mimic the three-
dimensionality of tumors. Several methods and an even larger number of protocols are …

The growth of tumour cells as three‐dimensional multicellular spheroids in vitro has led to
important insights in tumour biology, since properties of the in vivo‐tumour such as …

Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid
tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent …

Multicellular spheroids are three dimensional in vitro microscale tissue analogs. The current
article examines the suitability of spheroids as an in vitro platform for testing drug delivery …