In such a complicated field as proteomic analysis, scientists are more and more challenged
in implementing separation systems capable to provide enhanced separation power, as well …

This paper will review the challenges of developing a proteomics strategy. A key issue is the
integration of the two‐dimensional (2‐D) gel platform with mass spectrometry …

The central dogma of biology proposed that one gene encodes for one protein. We now
know that this does not reflect reality. The human body has approximately 20,000 protein …

In biomarker discovery, the detection of proteins with low abundance in the serum proteome
can be achieved by optimization of protein separation methods as well as selective …

Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key
components of current proteomics technology. The purpose of this review is to give an …

Current methods of proteome analysis rely almost solely on two‐dimensional
polyacrylamide gel electrophoresis (2‐D PAGE) followed by the excision of individual spots …

Mass spectrometry has become a primary tool for proteomics because of its capabilities for
rapid and sensitive protein identification and quantitation. It is now possible to identify …

A novel integrated multidimensional liquid chromatography (IMDL) method is demonstrated
for the separation of peptide mixtures by two-dimensional HPLC coupled with ion trap mass …

This review article is concerned with (i) proteomic sample preparation including among
other things the depletion of high‐abundance proteins and the concentration of low …

In this review we examine the current state of analytical methods in proteomics. The
conventional methodology using two-dimensional electrophoresis gels and mass …